Home LiteratureArticle Details
PMID: 6164984 Published · ppublish English Journal Article

Human fibroblast interferon gene lacks introns.

Nucleic acids research ·Vol. 9 ·No. 5 ·1981-03-11 ·Pages 1045-52

Lawn RM, Adelman J, Franke AE, Houck CM, Gross M, Najarian R, Goeddel DV

Abstract

A recombinant lambda bacteriophage isolated from a human genome library contains the gene for fibroblast interferon (IFN-beta1). The DNA sequence of this gene is identical to the sequence of its mRNA and is devoid of introns.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Fibroblasts/metabolism Humans Interferons/genetics
Chemicals
Interferons
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lawn R M
Adelman J
Franke A E
Houck C M
Gross M
Najarian R
Goeddel D V
References (28)
28 references, click to expand
  1. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.
    Biochim Biophys Acta. 1976 Sep 6;442(3):324-30 PMID: 183818
  2. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  3. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  6. Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
    Cell. 1978 Jul;14(3):655-71 PMID: 688387
  7. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  8. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  9. The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
    Cell. 1978 Nov;15(3):1033-44 PMID: 728984
  10. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  11. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  12. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  13. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence.
    Science. 1980 Feb 1;207(4430):525-6 PMID: 7352259
  14. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  15. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
    Nature. 1980 Mar 27;284(5754):316-20 PMID: 6987533
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. Isolation and structure of a human fibroblast interferon gene.
    Nature. 1980 Jun 19;285(5766):542-7 PMID: 6157094
  18. The nucleotide sequence of human fibroblast interferon cDNA.
    Gene. 1980 Jun;10(1):11-5 PMID: 6157601
  19. At least three human type alpha interferons: structure of alpha 2.
    Science. 1980 Sep 19;209(4463):1343-7 PMID: 6158094
  20. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
    Nature. 1980 Oct 2;287(5781):401-8 PMID: 6159536
  21. Human leukocyte interferon produced by E. coli is biologically active.
    Nature. 1980 Oct 2;287(5781):411-6 PMID: 6159538
  22. Heterogeneity of poly(I) x poly(C)-induced human fibroblast interferon mRNA species.
    Nature. 1980 Nov 6;288(5786):95-7 PMID: 6159547
  23. Synthesis of oligonucleotides on cellulose by a phosphotriester method.
    Nucleic Acids Res. 1980 May 24;8(10):2331-48 PMID: 7433092
  24. The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.
    Nucleic Acids Res. 1980 Jul 11;8(13):2885-94 PMID: 6159580
  25. Synthesis of human fibroblast interferon by E. coli.
    Nucleic Acids Res. 1980 Sep 25;8(18):4057-74 PMID: 6159584
  26. The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.
    Nucleic Acids Res. 1980 May 10;8(9):1913-31 PMID: 6159597
  27. NH2-terminal amino acid sequence of human fibroblast interferon.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5716-9 PMID: 6160579
  28. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.5.1045
Published
1981-03-11
Pages
1045-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326735
Subset
IM
Databases
GENBANK
J00218, K00616, M11029
External Links
PubMed source DOI: 10.1093/nar/9.5.1045 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2