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PMID: 6170068 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4557-61

Okuda K, Minami M, Ju ST, Dorf ME

Abstract

The serological characteristics of the antigen receptor on 4-hydroxy-3-nitrophenylacetyl (NP) specific suppressor T cell hybridomas were analyzed. Three T-cell hybrids could be lysed with anti-idiotype and complement. The reactivity pattern observed from a panel of anti-idiotypic reagents indicated that NPb determinants were detected on all three hybrid lines. NP conjugates with bovine serum albumin or caproic acid specifically inhibited the complement-mediated lysis of these cells by both anti-NPb idiotype and anti-I-J antisera. These hapten conjugates failed to block lysis by anti-Thy 1 or anti-H-2K antisera on the same target cell populations. The data indicate that both I-J and Igh variable region gene products are intimately involved in the recognition of antigen by suppressor T cells. Finally, the suppressor cell hybrids produce soluble factors that mediate antigen-specific suppression. The characteristics of the cells and their factors indicate that the hybrids correspond to the Tsi or first-order suppressor cells in the suppressor cell pathway.

MeSH Terms

Animals Cell Line Epitopes Haptens Hybridomas/immunology Immunoglobulin Idiotypes/immunology Isoantibodies Major Histocompatibility Complex Mice Nitrophenols/immunology Phenylacetates Receptors, Antigen, T-Cell/immunology T-Lymphocytes, Regulatory/immunology

Chemicals

Epitopes Haptens Immunoglobulin Idiotypes Isoantibodies Nitrophenols Phenylacetates Receptors, Antigen, T-Cell 4-hydroxy-5-nitrophenyl acetic acid

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Okuda K

Minami M

Ju S T

Dorf M E

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20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.7.4557

Published

1981-07-00

Pages

4557-61

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319831

Subset

Grants

NIAID NIH HHS · AI-16677 · United States

External Links

PubMed source DOI: 10.1073/pnas.78.7.4557 PMC Full Text View journal details

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Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells.

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