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PMID: 6170943 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA.

Nucleic acids research ·Vol. 9 ·No. 9 ·1981-05-11 ·Pages 2207-22

Bachellerie JP, Thompson JF, Wegnez MR, Hearst JE

Abstract

The reaction between RNA and 4'hydroxymethyl-4,5',8-trimethylpsoralen has been studied. Both natural RNA and synthetic RNAs were used. The base specificity of the reaction was found to be the same in natural RNA, homopolymers, and mononucleotides. Uridine was found to be the most reactive base in all cases. The kinetics of formation and reversal of monoadducts and crosslinks has been examined. Paper electrophoretic conditions are described which provide a separation of the monoaddition and crosslinked photoproducts. The relative and absolute amounts of monoadducts and crosslinks can be determined very accurately with this system. Paper electrophoresis provides good separations of the different photoproducts. The mobilities of the products are a simple function of their molecular weights and charges.

MeSH Terms
Chemical Phenomena Chemistry Drosophila/analysis Electrophoresis, Paper Endonucleases Endoribonucleases Escherichia coli/analysis Furocoumarins Kinetics Light Poly U RNA/radiation effects Ribonucleases Trioxsalen/analogs & derivatives Uridine
Chemicals
Furocoumarins Poly U hydroxymethyltrioxsalen RNA Endonucleases Endoribonucleases Ribonucleases ribonuclease T(2) Uridine Trioxsalen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bachellerie J P
Thompson J F
Wegnez M R
Hearst J E
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.9.2207
Published
1981-05-11
Pages
2207-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326836
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.9.2207 PMC Full Text View journal details
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