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PMID: 6171267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Developmental changes in the composition of polyadenylated RNA isolated from free and membrane-bound polyribosomes of the rat forebrain, analysed by translation in vitro.

The Biochemical journal ·Vol. 196 ·No. 1 ·1981-04-15 ·Pages 327-36

Hall C, Lim L

Abstract

Free and membrane-bound polyribosomes were isolated from the rat forebrain during its development. Polyadenylated RNA [poly(A)+ RNA] was isolated from both fractions, by using oligo(dT)-cellulose chromatography, and its composition studied by translating the poly(A)+ RNA in vitro in reticulocyte lysates. Electrophoretic analysis of the translation products showed that both free and membrane-bound polyribosomal poly(A)+ RNA gave many common components, but that there were also distinct differences in the protein composition of the products of the two fractions. Several proteins, of mol.wts. 39 000, 37 000, 31 000, 27 000 and 17 000, appeared to be products predominantly of free polyribosomal poly(A)+ RNA, whereas others, of mol.wt. 47 000, 33 000, 24 000 and 21 000 were specific to the membrane-bound polyribosomal poly(A)+ RNA fraction. More developmental changes were observed in the translational products of the membrane-bound poly(A)+ RNA fraction. Proteins of mol.wts. 33 000 and 21 000, which were predominant components of the translational products of this fraction when isolated from 10-day and older rats, were not present in translational products derived from preparations isolated from 3-day-old rats. The developmental appearance of these proteins as translational products of the membrane-bound poly(A)+ RNA suggests the appearance of new mRNA species. These transcriptional changes are discussed in relation to processes involved in brain differentiation, including myelination.

MeSH Terms
Animals Brain/growth & development,metabolism Cell Membrane/metabolism Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Female In Vitro Techniques Poly A/metabolism Polyribosomes/metabolism Protein Biosynthesis RNA/metabolism RNA, Messenger RNA, Ribosomal/metabolism Rats Reticulocytes/metabolism
Chemicals
RNA, Messenger RNA, Ribosomal Poly A RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hall C
Lim L
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47 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1960327
Published
1981-04-15
Pages
327-36
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162997
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1960327 PMC Full Text View journal details
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