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PMID: 271961 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Distribution of rat liver albumin mRNA membrane-bound and free in polyribosomes as determined by molecular hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5397-401

Yap SH, Strair RK, Shafritz DA

Abstract

Recently, we purified rat liver albumin mRNA and prepared albumin [(3)H]cDNA. Using albumin [(3)H]cDNA in molecular hybridization experiments, we have now determined the distribution of albumin mRNA sequences in membrane-bound and free liver polyribosomes prepared by techniques in which there is high recovery of polyribosomes without evidence of degradation. By using molecular hybridization to measure specific mRNA sequence content, numerous problems could be avoided in interpretation of results as obtained by cell-free protein synthesis or immunological methods. Under these conditions, 98% of albumin mRNA sequences in polyribosomes are found in the membrane-bound fraction.

MeSH Terms
Albumins/genetics Animals Base Sequence Cytoplasm/metabolism Endoplasmic Reticulum/metabolism Liver/metabolism,ultrastructure Male Poly A/metabolism Polyribosomes/metabolism RNA, Messenger/metabolism Rats
Chemicals
Albumins RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yap S H
Strair R K
Shafritz D A
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5397
Published
1977-12-00
Pages
5397-401
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431734
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5397 PMC Full Text View journal details
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