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PMID: 986643 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

An assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ.

Nucleic acids research ·Vol. 3 ·No. 6 ·1976-06-00 ·Pages 1427-36

Peterson JA

Abstract

The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.

MeSH Terms
Animals Leucine/metabolism Liver Magnesium/pharmacology Potassium/pharmacology Protein Biosynthesis RNA, Messenger/metabolism RNA, Ribosomal/metabolism Rats Serum Albumin/biosynthesis Triticum/metabolism
Chemicals
RNA, Messenger RNA, Ribosomal Serum Albumin Leucine Magnesium Potassium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Peterson J A
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.6.1427
Published
1976-06-00
Pages
1427-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343002
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.6.1427 PMC Full Text View journal details
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