Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining.

Applied and environmental microbiology ·Vol. 32 ·No. 1 ·1976-07-00 ·Pages 159-65

Apel WA, Dugan PR, Filppi JA, Rheins MS

Abstract

An indirect fluorescent antibody (FA) staining technique was developed for the rapid detection of Thiobacillus ferrooxidans. The specificity of the FA stain for T. ferrooxidans was demonstrated with both laboratory and environmental samples. Coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. Significant numbers of T. ferrooxidans were detected in the refuse pores. A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique.

MeSH Terms

Coal Mining Epitopes Fluorescent Antibody Technique Industrial Waste Soil Microbiology Thiobacillus/immunology,isolation & purification

Chemicals

Epitopes Industrial Waste

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Apel W A

Dugan P R

Filppi J A

Rheins M S

References (17)

17 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.32.1.159-165.1976

Published

1976-07-00

Pages

159-65

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC170021

Subset

External Links

PubMed source DOI: 10.1128/aem.32.1.159-165.1976 PMC Full Text View journal details

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Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining.

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