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PMID: 6174950 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 7013-32

Torelli G, Ferrari S, Donelli A, Cadossi R, Ferrari S, Bosi P, Torelli U

Abstract

The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduced in respect to normal unstimulated lymphocytes. The differences is due to a small portion, by weight, of high complexity sequences, suggesting the involvement or nuclear, not necessarily transcriptional, events. Moreover the heterologous hybridizations show that: 1) in PHA-stimulated lymphocytes there is a set of highly abundant sequences which are represented in normal and leukemic lymphocytes at a very reduced rate; 2) the sequences abundant in normal unstimulated lymphocytes are less represented in leukemic lymphocytes; the converse also is true, but at a lower extent. On the basis of available data, it is suggested that the observed changes in the abundance pattern of PHA-stimulated lymphocytes may be related mainly to a difference in the half-life of the mRNAs, while those of leukemic lymphocytes require some transcriptional or post-transcriptional nuclear event.

MeSH Terms
Base Sequence Cell Nucleus/metabolism Cells, Cultured Humans Leukemia, Lymphoid/genetics Lymphocyte Activation Lymphocytes/metabolism Nucleic Acid Hybridization Phytohemagglutinins/pharmacology Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger RNA, Neoplasm/genetics Transcription, Genetic
Chemicals
Phytohemagglutinins RNA, Messenger RNA, Neoplasm Poly A RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Torelli G
Ferrari S
Donelli A
Cadossi R
Ferrari S
Bosi P
Torelli U
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.24.7013
Published
1981-12-21
Pages
7013-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327658
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.24.7013 PMC Full Text View journal details
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