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PMID: 6179502 Published · ppublish English Journal Article

Isolation and characterization of a canine rotavirus.

Archives of virology ·Vol. 72 ·No. 1-2 ·1982-00-00 ·Pages 113-25

Hoshino Y, Wyatt RG, Scott FW, Appel MJ

Abstract

Canine rotavirus particles were visualized by direct electron microscopy in the feces from a clinically normal dog. The virus was subsequently propagated in cell cultures; it was characterized and compared with rotaviruses from other species. Replication of the virus in cell culture was found to be less dependent upon trypsin than that of human, bovine and porcine rotaviruses. Reproducible, sharp-edged plaques of various sizes were produced by the canine rotavirus in an established cell line of fetal rhesus monkey kidney, MA 104, under overlays of carboxymethyl cellulose or agarose. Intracytoplasmic inclusion bodies of different sizes and shapes were produced in infected MA 104 cells. By plaque reduction neutralization assay, a two-way antigenic relationship was found between the canine (CU-1) and simian (rhesus MMU 18006 and SA-11) rotaviruses. The canine rotavirus had a one-way antigenic relationship with feline (Taka), bovine (NCDV), and porcine (OSU) rotaviruses.

MeSH Terms
Animals Antigens, Viral/immunology Cell Line Cytopathogenic Effect, Viral Dogs/microbiology Epitopes Feces/microbiology Humans Inclusion Bodies, Viral/ultrastructure Mammals/microbiology Reoviridae/isolation & purification Rotavirus/immunology,isolation & purification,physiology Species Specificity Trypsin/pharmacology Viral Plaque Assay
Chemicals
Antigens, Viral Epitopes Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hoshino Y
Wyatt R G
Scott F W
Appel M J
References (48)
48 references, click to expand
  1. The effect of trypsin on the growth of rotavirus.
    J Gen Virol. 1978 Jul;40(1):213-8 PMID: 211180
  2. Studies on the effect of chymotrypsin on reovirions.
    Virology. 1972 Sep;49(3):700-15 PMID: 4672416
  3. Isolation and cell culture propagation of rotaviruses from turkeys and chickens.
    Arch Virol. 1979;61(1-2):13-21 PMID: 92979
  4. Rotavirus infection in commercial laying hens.
    Vet Rec. 1979 Jan 6;104(1):22 PMID: 219583
  5. Canine viral enteritis. Recent developments.
    Mod Vet Pract. 1979 May;60(5):375-80 PMID: 224304
  6. Human rotavirus in young dogs.
    Med J Aust. 1976 Dec 11;2(24):922-3 PMID: 190523
  7. Effect of enzymes on rotavirus infectivity.
    J Clin Microbiol. 1979 Jul;10(1):111-3 PMID: 227917
  8. Viral intestinal infections of animals and man.
    Comp Immunol Microbiol Infect Dis. 1979;2(4):417-35 PMID: 232863
  9. Canine viral enteritis: Prevalence of parvo-, corona- and rotavirus infections in dogs in the Netherlands.
    Vet Q. 1980 Oct;2(4):181-90 PMID: 22039978
  10. Diarrhea caused in gnotobiotic piglets by the reovirus-like agent of human infantile gastroenteritis.
    J Infect Dis. 1976 Jan;133(1):22-7 PMID: 173760
  11. Approaches to immunization of infants and young children against gastroenteritis due to rotaviruses.
    Rev Infect Dis. 1980 May-Jun;2(3):459-69 PMID: 6251528
  12. Structure of the bluetongue virus capsid.
    J Virol. 1972 Oct;10(4):783-94 PMID: 4117349
  13. Cell culture propagation of porcine rotavirus (reovirus-like agent).
    Am J Vet Res. 1977 Nov;38(11):1765-8 PMID: 201198
  14. Rotavirus infection in avian species.
    Vet Rec. 1978 Sep 30;103(14):319-20 PMID: 213873
  15. Propagation of bovine rotavirus by dogs.
    Vet Rec. 1981 Aug 29;109(9):187 PMID: 6275602
  16. Diarrhea in gnotobiotic calves caused by the reovirus-like agent of human infantile gastroenteritis.
    Infect Immun. 1976 Aug;14(2):471-4 PMID: 184047
  17. A plaque assay for the simian rotavirus SAII.
    J Gen Virol. 1979 Jun;43(3):513-9 PMID: 225432
  18. Simian virus SA11 and the related O agent.
    Arch Gesamte Virusforsch. 1967;22(1):235-45 PMID: 4972061
  19. Cell culture propagation of neonatal calf diarrhea (scours) virus.
    Can Vet J. 1971 Mar;12(3):69-72 PMID: 4933992
  20. Rotavirus (reovirus-like) infection of neonatal ruminants in a zoo nursery.
    J Wildl Dis. 1978 Jul;14(3):351-4 PMID: 211273
  21. Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):420-4 PMID: 6264442
  22. Isolation and characterization of feline rotavirus.
    J Gen Virol. 1981 Jun;54(Pt 2):313-23 PMID: 6270251
  23. Production of high-titer bovine rotavirus with trypsin.
    J Clin Microbiol. 1979 Mar;9(3):413-7 PMID: 222801
  24. Proteolytic enhancement of rotavirus infectivity: biology mechanism.
    Virology. 1980 Mar;101(2):432-9 PMID: 6244698
  25. A morphological study of human rotavirus.
    Arch Virol. 1979;61(1-2):41-8 PMID: 229794
  26. Cell culture studies of a neonatal calf diarrhea virus.
    Can J Comp Med. 1973 Jul;37(3):287-94 PMID: 4270436
  27. Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus.
    Science. 1979 Feb 9;203(4380):548-50 PMID: 216077
  28. Viral gastroenteritis.
    Prog Med Virol. 1979;25:1-36 PMID: 228348
  29. Rotaviruses in diarrheic feces of a dog.
    Vet Med Small Anim Clin. 1979 Jun;74(6):817-9 PMID: 223269
  30. ENTERIC cytopathogenic human orphan (ECHO) viruses.
    Science. 1955 Dec 16;122(3181):1187-8 PMID: 13274079
  31. Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus.
    J Clin Microbiol. 1979 Apr;9(4):488-92 PMID: 222805
  32. Coronavirus-like particles in the feces of normal cats.
    Arch Virol. 1980;63(2):147-52 PMID: 6986859
  33. Viruses and diarrhoea in dogs.
    Vet Rec. 1980 Apr 12;106(15):350-1 PMID: 6246671
  34. Propagation of infantile gastroenteritis virus (orbi-group) in conventional and germfree piglets.
    Infect Immun. 1975 Dec;12(6):1276-80 PMID: 1239420
  35. Human rotavirus type 2: cultivation in vitro.
    Science. 1980 Jan 11;207(4427):189-91 PMID: 6243190
  36. Proteolytic enzymes and rotavirus SA-11 plaque formation.
    Can J Comp Med. 1980 Apr;44(2):232-6 PMID: 6250685
  37. Electron microscopy detection and characterization of viral particles in dog stools.
    Arch Virol. 1980;66(2):89-93 PMID: 6254477
  38. Antigenic comparisons of two new rotaviruses from rhesus monkeys.
    J Clin Microbiol. 1980 Feb;11(2):202-3 PMID: 6244332
  39. Transmission of human rotaviruses to gnotobiotic piglets.
    J Med Microbiol. 1975 Nov;8(4):565-9 PMID: 1107554
  40. Human rotavirus in lambs: infection and passive protection.
    Infect Immun. 1977 Apr;16(1):268-70 PMID: 194837
  41. Isolation of a rotavirus from a newborn dog with diarrhea.
    Am J Vet Res. 1981 May;42(5):841-3 PMID: 6266295
  42. Antibody to rotavirus in various animal species.
    Natl Inst Anim Health Q (Tokyo). 1979 Summer;19(1-2):72-3 PMID: 231743
  43. The rotaviruses.
    Arch Virol. 1978;57(1):1-23 PMID: 77663
  44. Rotaviruses.
    J Gen Virol. 1978 Jul;40(1):1-18 PMID: 80441
  45. Simian rotavirus SA-11 plaque formation in the presence of trypsin.
    J Clin Microbiol. 1979 Nov;10(5):609-14 PMID: 94597
  46. Electron microscopic identification and subsequent isolation of a rotavirus from a dog with fatal neonatal diarrhea.
    Am J Vet Res. 1980 May;41(5):782-3 PMID: 6250431
  47. Rotavirus isolation and cultivation in the presence of trypsin.
    J Clin Microbiol. 1977 Dec;6(6):610-7 PMID: 201663
  48. Antibody to rotavirus in dogs and cats.
    Vet Rec. 1978 Jun 17;102(24):534-5 PMID: 209605
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01314456
Published
1982-00-00
Pages
113-25
Language
English
Region
Austria
NLM ID
7506870
PMCID
PMC7087106
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01314456 PMC Full Text View journal details
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