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PMID: 6185953 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specificity of antibodies elicited by a synthetic peptide having a sequence in common with a fragment of a virus protein, the hepatitis B surface antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 24 ·1982-12-00 ·Pages 7871-5

Neurath AR, Kent SB, Strick N

Abstract

We predicted the localization of a major hepatitis B surface antigen (HBsAg) determinant within residues 135-155 [Neurath, A.R., Strick, N. & Oleszko, W.R. (1981) J. Virol. Methods 3, 115-125]. A peptide corresponding to this sequence (P135-155) was synthesized, linked to macromolecular carriers, and used to immunize rabbits. In accordance with published data on shorter peptides within the same amino acid sequence, antibodies to HBsAg were elicited. A detailed analysis of the immune response to P135-155 revealed the following: A heterogeneous population of IgG and IgM antibodies reacting with P135-155 was detected in the antisera by a variety of radioimmunoassays and enzyme-linked fluorescence immunoassays. Only a subpopulation of these antibodies reacted with HBsAg. The equilibrium constant (K) for the reaction of the antibodies with HBsAg (K = 4 X 10(5) M-1) was approximately two orders of magnitude lower than K for the reaction with P135-155 and was below K for the reaction between HBsAg and antibodies elicited by HBsAg (K greater than 10(7) M-1). Preimmunization with P135-155 did not result in an enhanced response to subsequent immunization with HBsAg. Peptides more accurately mimicking determinants on HBsAg may have to be synthesized for possible application in antiviral prophylaxis.

MeSH Terms
Amino Acid Sequence Antibodies, Viral/immunology Antibody Specificity Epitopes Hepatitis B Antibodies/immunology Hepatitis B Surface Antigens/immunology Peptide Fragments/immunology Viral Vaccines
Chemicals
Antibodies, Viral Epitopes Hepatitis B Antibodies Hepatitis B Surface Antigens Peptide Fragments Viral Vaccines
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Neurath A R
Kent S B
Strick N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.24.7871
Published
1982-12-00
Pages
7871-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347451
Subset
IM
Grants
PHS HHS · 09011 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.24.7871 PMC Full Text View journal details
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