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PMID: 6190745 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transformation of LMTK- cells with purified HLA class I genes. II. Serologic characterization of HLA-A3 and CW3 molecules.

Immunogenetics ·Vol. 18 ·No. 1 ·1983-00-00 ·Pages 65-77

Lemonnier FA, Dubreuil PC, Layet C, Malissen M, Bourel D, Mercier P, Jakobsen BK, Caillol DH, Svejgaard A, Kourilsky FM, Jordan BR

Abstract

The expression of two different HLA class I genes was observed after transformation of LMTK- cells. The corresponding class I molecules reacted differentially with monomorphic monoclonal antibodies (m.Ab). Absorption and elution studies of the human alloantibodies reacting with the transformed cells and cellular radioimmunoassay of these cells with polymorphic m.Ab resulted in the identification of HLA-A3 and CW3 molecules. These transformed cells were used to immunize C3H mice and induce the production of xenogeneic antisera, which, following absorption, showed polymorphic reactivity with human cells, suggesting that some of these sera could be used as typing reagents.

MeSH Terms
Animals Antibodies, Monoclonal DNA Restriction Enzymes DNA Transposable Elements Epitopes/analysis Genes HLA Antigens/genetics HLA-A3 Antigen HLA-C Antigens L Cells/enzymology,immunology Major Histocompatibility Complex Mice Mice, Inbred BALB C Radioimmunoassay Species Specificity Thymidine Kinase/deficiency,genetics
Chemicals
Antibodies, Monoclonal DNA Transposable Elements Epitopes HLA Antigens HLA-A3 Antigen HLA-C Antigens HLA-C*03 antigen Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Lemonnier F A
Dubreuil P C
Layet C
Malissen M
Bourel D
Mercier P
Jakobsen B K
Caillol D H
Svejgaard A
Kourilsky F M
Jordan B R
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00401357
Published
1983-00-00
Pages
65-77
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00401357 View journal details
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