Home LiteratureArticle Details
PMID: 6195282 Published · ppublish English Journal Article

Galactan-binding antibodies. Diversity and structure of idiotypes.

The Journal of experimental medicine ·Vol. 158 ·No. 5 ·1983-11-01 ·Pages 1385-400

Rudikoff S, Pawlita M, Pumphrey J, Mushinski E, Potter M

Abstract

A group of eight IgM hybridoma proteins induced with beta(1,6)-D-galactan-containing antigens has been characterized in terms of primary amino acid sequence and idiotype expression. The H chain amino acid sequences reveal very strong homology in the VH segment although several substitutions are seen that suggest the occurrence of somatic mutation in these IgM molecules. Significant sequence variation was observed in CDR-3, the region generated by the D segment, and the two recombination events, VH-D and D-JH. The number of amino acids in this region contributed by the D segment was found to vary from two to six, yet the overall length of CDR-3 was precisely maintained by the addition of amino acids on either side of D during the recombination processes. These additional amino acids are suggested to result from nucleotide addition by repair enzymes. Idiotypic analysis of these proteins, in conjunction with an assessment of the H chain sequences, has permitted an identification of the molecular basis of both cross-reacting and unique idiotypic determinants expressed by these molecules.

MeSH Terms
Amino Acid Sequence Animals Antibodies/immunology Epitopes/immunology Galactans/immunology Hybridomas/immunology Immunoglobulin Heavy Chains Immunoglobulin Idiotypes/immunology Immunoglobulin Variable Region Mice Multiple Myeloma/immunology
Chemicals
Antibodies Epitopes Galactans Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rudikoff S
Pawlita M
Pumphrey J
Mushinski E
Potter M
References (48)
48 references, click to expand
  1. Variability in the lambda light chain sequences of mouse antibody.
    Nature. 1970 Dec 12;228(5276):1045-7 PMID: 5483159
  2. A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.
    Cell. 1981 Jul;25(1):59-66 PMID: 6791833
  3. Spectral changes on binding of oligosaccharides to murine immunoglobulin A myeloma proteins.
    Biochemistry. 1973 Jul 31;12(16):3039-44 PMID: 4581145
  4. Structural requirements for the binding of derivatives of D-galactose to two homogeneous murine immunoglobulins.
    Biochemistry. 1974 Jul 16;13(15):3179-84 PMID: 4601801
  5. Towards a network theory of the immune system.
    Ann Immunol (Paris). 1974 Jan;125C(1-2):373-89 PMID: 4142565
  6. Subunit interactions in mouse myeloma proteins with anti-galactan activity.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):932-6 PMID: 815909
  7. The structure and genetics of mouse immunoglobulins: an analysis of NZB myeloma proteins and sets of BALB/c myeloma proteins binding particular haptens.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:817-36 PMID: 330088
  8. Restriction in IgM expression--I. The VH regions of equine anti-lactose antibodies.
    Mol Immunol. 1980 Dec;17(12):1553-61 PMID: 6795450
  9. Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
    Nucleic Acids Res. 1981 Aug 25;9(16):4099-109 PMID: 6795591
  10. A hypothetical space-filling model of the V-regions of the galactan-binding myeloma immunoglobulin J539.
    Mol Immunol. 1981 Aug;18(8):683-98 PMID: 6171725
  11. The complete V domain amino acid sequences of two myeloma inulin-binding proteins.
    J Immunol. 1982 Jan;128(1):302-7 PMID: 6798111
  12. A monoclonal antibody that defines an idiotope with two subsites in galactan-binding myeloma proteins.
    J Exp Med. 1981 Dec 1;154(6):1946-56 PMID: 7320687
  13. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.
    J Exp Med. 1982 Jan 1;155(1):201-18 PMID: 6798155
  14. Somatic mutation in a cultured mouse myeloma cell affects antigen binding.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1240-4 PMID: 6803237
  15. Single amino acid substitution altering antigen-binding specificity.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1979-83 PMID: 6804947
  16. kappa-Chain restriction in anti-galactan antibodies.
    J Immunol. 1982 Aug;129(2):615-8 PMID: 6806376
  17. Polymorphism in immunoglobulin heavy chains suggesting gene conversion.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3280-4 PMID: 6808507
  18. Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes.
    J Mol Appl Genet. 1981;1(2):103-16 PMID: 6286818
  19. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118-22 PMID: 6287467
  20. Polymorphism in anti-phosphocholine antibodies reflecting evolution of immunoglobulin families.
    J Exp Med. 1983 Jan 1;157(1):98-113 PMID: 6401319
  21. Evolution of immunoglobulin V genes: evidence indicating that recently duplicated human V kappa sequences have diverged by gene conversion.
    Cell. 1983 Jan;32(1):181-9 PMID: 6402305
  22. An idiotypic determinant formed by both immunoglobulin constant and variable regions.
    Nature. 1983 Feb 24;301(5902):720-2 PMID: 6186921
  23. Immunoglobulin structure--function correlates: antigen binding and idiotypes.
    Contemp Top Mol Immunol. 1983;9:169-209 PMID: 6347517
  24. Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes.
    EMBO J. 1982;1(5):635-40 PMID: 7188353
  25. Immunological relationships among the myeloma proteins.
    J Exp Med. 1955 Jan 1;101(1):85-108 PMID: 13211928
  26. Idiotypes on galactan binding myeloma proteins and anti-galactan antibodies in mice.
    J Immunol. 1977 Dec;119(6):1888-93 PMID: 72109
  27. Antigen-binding myeloma proteins of mice.
    Adv Immunol. 1977;25:141-211 PMID: 76433
  28. Organization of immunoglobulin heavy chain genes and allelic deletion model.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2140-4 PMID: 97665
  29. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  30. Rearrangement of genetic information may produce immunoglobulin diversity.
    Nature. 1978 Dec 21-28;276(5690):785-90 PMID: 103003
  31. The structural basis of a hapten-inhibitable kappa-chain idiotype.
    J Immunol. 1979 May;122(5):1905-10 PMID: 109517
  32. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  33. Structural evidence for independent joining region gene in immunoglobulin heavy chains from anti-galactan myeloma proteins and its potential role in generating diversity in complementarity-determining regions.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2890-4 PMID: 111245
  34. Structural and genetic basis of idiotypy in the galactan-binding myeloma proteins.
    Ann Immunol (Paris). 1979 Mar-Apr;130(2):263-71 PMID: 313744
  35. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  36. Crystal structure of galactan-binding mouse immunoglobulin J539 Fab at 4.5-A resolution.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):4071-4 PMID: 291062
  37. The joining of V and J gene segments creates antibody diversity.
    Nature. 1980 Jan 31;283(5746):497-9 PMID: 6766210
  38. An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
    Nature. 1980 Feb 21;283(5749):733-9 PMID: 6766532
  39. Structural correlates of cross-reactive and individual idiotypic determinants on murine antibodies to alpha-(1 leads to 3) dextran.
    J Exp Med. 1980 May 1;151(5):1059-70 PMID: 6154766
  40. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  41. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  42. Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome.
    Nature. 1980 Aug 28;286(5776):850-3 PMID: 6774261
  43. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630-4 PMID: 6251474
  44. kappa Chain joining segments and structural diversity of antibody combining sites.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4270-4 PMID: 6776525
  45. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.
    Nature. 1981 Apr 16;290(5807):562-5 PMID: 6783961
  46. Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):509-13 PMID: 6787590
  47. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  48. Mouse lambda-chain sequences.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2112-6 PMID: 4516208
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.158.5.1385
Published
1983-11-01
Pages
1385-400
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187133
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.158.5.1385 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2