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PMID: 6196192 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.

The EMBO journal ·Vol. 2 ·No. 11 ·1983-00-00 ·Pages 1883-91

Mattaj IW, Zeller R

Abstract

Xenopus laevis U2 small nuclear RNA (snRNA) genes were isolated and expressed by microinjection into frog oocytes. The genes are organised in short tandemly repeated units of approximately 830 bp. Some of the cloned tandem repeats are closely linked to genes coding for U5 snRNA, tRNA and an uncharacterised 7S RNA. No evidence was found for U2 snRNA pseudogenes. Single repeat units are transcriptionally active, showing that all the signals necessary for U2 snRNA transcription are included in an 831-bp segment of DNA. Sequence analysis of a cloned repeat unit showed that Xenopus and rat U2 snRNAs are 94% homologous. Flanking regions 5' and 3' to the coding sequence were found which shared extensive homology with similarly positioned sequences in human U1 snRNA genes. Part of the 3' non-coding region homology (consensus TTTNAAAGAAT) was found in many other genes transcribed by RNA polymerase II.

MeSH Terms
Animals Base Sequence Biological Evolution Genes Nucleic Acid Conformation RNA/genetics RNA Polymerase II/genetics RNA, Small Nuclear Repetitive Sequences, Nucleic Acid Transcription, Genetic Xenopus laevis/genetics
Chemicals
RNA, Small Nuclear RNA RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mattaj I W
Zeller R
References (36)
36 references, click to expand
  1. Dictyostelium small nuclear RNA D2 is homologous to rat nucleolar RNA U3 and is encoded by a dispersed multigene family.
    Cell. 1980 Nov;22(1 Pt 1):109-18 PMID: 6159098
  2. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  3. The primary transcription unit of the mouse beta-major globin gene.
    Cell. 1981 Feb;23(2):585-93 PMID: 6937266
  4. Structure and expression of a chicken gene coding for U1 RNA.
    Cell. 1981 Mar;23(3):671-80 PMID: 6164492
  5. Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):810-4 PMID: 6165010
  6. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2737-41 PMID: 6789322
  7. The organization and expression of histone gene families.
    Cell. 1981 Aug;25(2):301-13 PMID: 6793234
  8. Primary and secondary structure of U2 snRNA.
    Nucleic Acids Res. 1981 Nov 11;9(21):5645-58 PMID: 6796940
  9. Direct repeats flank three small nuclear RNA pseudogenes in the human genome.
    Cell. 1981 Oct;26(1 Pt 1):11-7 PMID: 6173130
  10. Intracellular transport of microinjected 5S and small nuclear RNAs.
    Nature. 1982 Feb 18;295(5850):572-7 PMID: 6173771
  11. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  12. Sea urchin small nuclear RNA genes are organized in distinct tandemly repeating units.
    Nucleic Acids Res. 1982 Dec 11;10(23):7677-88 PMID: 6185926
  13. The Xenopus laevis globin gene family: chromosomal arrangement and gene structure.
    Cell. 1983 Jan;32(1):45-53 PMID: 6825173
  14. Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis.
    Cell. 1983 Feb;32(2):425-34 PMID: 6186395
  15. Structural analysis of gene loci for rat U1 small nuclear RNA.
    Nucleic Acids Res. 1983 Mar 25;11(6):1791-801 PMID: 6188110
  16. Nucleocytoplasmic segregation of proteins and RNAs.
    Cell. 1983 Apr;32(4):1021-5 PMID: 6188533
  17. Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B.
    J Mol Biol. 1983 Mar 25;165(1):35-57 PMID: 6302284
  18. Genetic analysis of the processing of a spliced tRNA.
    EMBO J. 1982;1(2):263-8 PMID: 6926726
  19. U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
    EMBO J. 1982;1(10):1259-65 PMID: 6202507
  20. Cloning and structure of the human immune interferon-gamma chromosomal gene.
    EMBO J. 1982;1(8):953-8 PMID: 6329718
  21. The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):428-32 PMID: 1054825
  22. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  23. Isolation and some properties of DNA coding for tRNA1met from Xenopus laevis.
    Cell. 1976 Jun;8(2):183-95 PMID: 986875
  24. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  25. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  26. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  27. Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA.
    Nucleic Acids Res. 1978 Mar;5(3):905-24 PMID: 347404
  28. The transcription of 5 S DNA injected into Xenopus oocytes.
    Dev Biol. 1978 Dec;67(2):346-56 PMID: 738532
  29. A regulatory sequence near the 3' end of sea urchin histone genes.
    Nucleic Acids Res. 1979 Jul 11;6(9):2997-3008 PMID: 493132
  30. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1437-41 PMID: 6445556
  31. Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
    Cell. 1982 Apr;28(4):739-45 PMID: 6284372
  32. Human U1 loci: genes for human U1 RNA have dramatically similar genomic environments.
    Cell. 1982 May;29(1):257-64 PMID: 6179629
  33. Transcription of a gene for human U1 small nuclear RNA.
    Cell. 1982 May;29(1):265-74 PMID: 6179630
  34. SnRNAs, SnRNPs, and RNA processing.
    Annu Rev Biochem. 1982;51:617-54 PMID: 6180681
  35. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
    Cell. 1982 Oct;30(3):675-86 PMID: 6183003
  36. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1883-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555384
Subset
IM
Databases
GENBANK
X00093
External Links
PubMed source PMC Full Text View journal details
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