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PMID: 6198648 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 2 ·1984-01-00 ·Pages 419-23

Ward WS, Schmidt WN, Schmidt CA, Hnilica LS

Abstract

The relationship between the rat tumor-associated cytokeratin p39 (Mr 39,000) and cellular DNA has been studied in intact cells. Using a DNA-protein crosslinking technique, incubation with cis-dichlorodiammineplatinum, we present evidence for the association of p39 with DNA in intact Novikoff ascites hepatoma cells. The cells were treated with cis-dichlorodiammineplatinum and solubilized in NaDodSO4-containing buffer, and the DNA was pelleted by high-speed centrifugation. By immunotransfer analysis, the cytokeratin was found in the DNA pellet of the crosslinked samples while absent from the controls. This result was further substantiated by CsCl density-gradient centrifugation. Collectively, these results suggest a cytokeratin-DNA association at the filament binding sites on or near the nuclear lamina.

MeSH Terms
Animals Antigens, Neoplasm/metabolism Cell Nucleus/ultrastructure Cisplatin DNA/metabolism Histones/metabolism Intermediate Filament Proteins/metabolism Keratins/metabolism Liver Neoplasms, Experimental/metabolism Molecular Weight Protein Binding Rats
Chemicals
Antigens, Neoplasm Histones Intermediate Filament Proteins Keratins DNA Cisplatin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ward W S
Schmidt W N
Schmidt C A
Hnilica L S
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.2.419
Published
1984-01-00
Pages
419-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344688
Subset
IM
Grants
NCI NIH HHS · CA 26412 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.2.419 PMC Full Text View journal details
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