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PMID: 6201721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of 5-azacytidine on methylation and expression of specific DNA sequences in C3H 10T1/2 cells.

Molecular and cellular biology ·Vol. 4 ·No. 4 ·1984-04-00 ·Pages 634-41

Hsiao WL, Gattoni-Celli S, Kirschmeier P, Weinstein IB

Abstract

The present study indicates that the transient exposure of C3H 10T1/2 mouse embryo fibroblasts to 5-azacytidine leads to extensive loss of methylation of the protooncogene c-mos and the beta-globin locus at the cell population level and in at least 40 isolated subclones. These changes persisted, even when the cells were serially passaged for many generations without further exposure to the drug. Even though the amount of demethylation, assessed through differential digestion by the restriction enzymes HpaII and MspI, was quite extensive, neither locus was transcribed at a detectable level. This nonselective analysis suggests, therefore, that loss of DNA methylation is not sufficient per se to induce the expression of certain loci. Presumably, the expression of these loci requires additional factors, some of which may be related to cell lineage and differentiation.

MeSH Terms
Animals Azacitidine/pharmacology Cell Line Clone Cells/physiology Gene Expression Regulation/drug effects Globins/genetics Methylation Mice Mice, Inbred C3H Oncogenes
Chemicals
Globins Azacitidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsiao W L
Gattoni-Celli S
Kirschmeier P
Weinstein I B
References (33)
33 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Rearranged c-mos locus in a MOPC 21 murine myeloma cell line and its persistence in hybridomas.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):795-6 PMID: 6656882
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. Correlation between hypomethylation of DNA and expression of globin genes in Friend erythroleukemia cells.
    Eur J Biochem. 1977 Nov 15;81(1):53-61 PMID: 271073
  5. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
    Cell. 1978 Apr;13(4):717-25 PMID: 566164
  6. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.
    Nucleic Acids Res. 1978 Dec;5(12):4631-4 PMID: 745990
  7. Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.
    Nature. 1979 Aug 2;280(5721):419-20 PMID: 460418
  8. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
    Cell. 1979 Aug;17(4):771-9 PMID: 90553
  9. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  10. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  11. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  12. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
    Nucleic Acids Res. 1979 Dec 20;7(8):2081-103 PMID: 523314
  13. Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus.
    Science. 1980 Mar 14;207(4436):1222-4 PMID: 6243788
  14. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.
    Cell. 1980 Apr;19(4):947-58 PMID: 6247075
  15. Cellular differentiation, cytidine analogs and DNA methylation.
    Cell. 1980 May;20(1):85-93 PMID: 6156004
  16. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  17. DNA methylation and gene function.
    Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
  18. Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6634-8 PMID: 6935674
  19. Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation.
    Science. 1981 Jan 23;211(4480):393-6 PMID: 6164095
  20. Activation of the transforming potential of a normal cell sequence: a molecular model for oncogenesis.
    Science. 1981 May 22;212(4497):941-3 PMID: 7233190
  21. DNA sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):95-9 PMID: 6264457
  22. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
    Nature. 1981 Jul 23;292(5821):311-7 PMID: 6166864
  23. DNA methylation controls the inducibility of the mouse metallothionein-I gene lymphoid cells.
    Cell. 1981 Jul;25(1):233-40 PMID: 6168387
  24. 5-Azacytidine induction of mouse endogenous type C virus and suppression of DNA methylation.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6290-4 PMID: 6171816
  25. Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2'-deoxycytidine.
    J Biol Chem. 1982 Feb 25;257(4):2041-8 PMID: 6173384
  26. Pre-adipocyte determination either by insulin or by 5-azacytidine.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):480-4 PMID: 6176995
  27. Carcinogen- and radiation-transformed C3H 10T1/2 cells contain RNAs homologous to the long terminal repeat sequence of a murine leukemia virus.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2773-7 PMID: 6178111
  28. Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells.
    Mol Cell Biol. 1982 Jan;2(1):42-51 PMID: 6287220
  29. Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element.
    Nature. 1982 Dec 16;300(5893):607-11 PMID: 6292737
  30. Intracisternal A-particle genes as movable elements in the mouse genome.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992-6 PMID: 6300886
  31. The sequence GGCmCGG is resistant to MspI cleavage.
    Nucleic Acids Res. 1983 Jun 11;11(11):3559-69 PMID: 6190135
  32. Effect of CpG methylation on Msp I.
    Nucleic Acids Res. 1983 Jun 11;11(11):3571-80 PMID: 6190136
  33. Nucleotide sequences in mouse DNA and RNA specific for Moloney sarcoma virus.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3705-9 PMID: 185623
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.4.634-641.1984
Published
1984-04-00
Pages
634-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368771
Subset
IM
Grants
NCI NIH HHS · CA-02111 · United States
NIGMS NIH HHS · GM07088-07 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.4.634-641.1984 PMC Full Text View journal details
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