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PMID: 6212756 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The cis-specificity of the Q-gene product of bacteriophage lambda.

Molecular & general genetics : MGG ·Vol. 185 ·No. 3 ·1982-00-00 ·Pages 468-72

Burt DW, Brammar WJ

Abstract

A trp/lacW205 substitution, fused to the late region of bacteriophage lambda, provided a convenient assay for phage late gene expression in the presence or absence of lambda pQ. Comparison of lacZ expression from Q+ and Q- phages showed that late gene expression was markedly Q-dependent (263-fold difference). A cis/trans comparison of lambda pQ action showed a 180-fold difference in lacZ expression. The results suggest that pQ in only significantly active when supplied in cis to its site of action.

MeSH Terms
Bacteriophage lambda/genetics Crosses, Genetic Escherichia coli/genetics Genes, Viral Genotype Phenotype Recombination, Genetic Species Specificity Transcription, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burt D W
Brammar W J
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334142
Published
1982-00-00
Pages
468-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334142
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