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PMID: 6285144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional termination sites in the b2 region of bacteriophage lambda that are unresponsive to antitermination.

Molecular & general genetics : MGG ·Vol. 185 ·No. 3 ·1982-00-00 ·Pages 462-7

Burt DW, Brammar WJ

Abstract

A bacteriophage lambda cloning vector carrying the trp/lacW205 substitution is described. The vector facilitates the fusion in vitro of genetic control signals to the lacZ structural gene of Escherichia coli. This system was used to define transcriptional termination sites in the lambda b2 region. This region contains termination sites that are unresponsive to the lambda antiterminating proteins pQ and pN.

MeSH Terms
Bacteriophage lambda/genetics Cloning, Molecular Crosses, Genetic DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Genes Genotype Kinetics Phenotype Transcription, Genetic beta-Galactosidase/genetics
Chemicals
DNA, Viral DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burt D W
Brammar W J
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334141
Published
1982-00-00
Pages
462-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334141
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