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PMID: 6244540 Published · ppublish English Journal Article

Reovirus-induced modification of cap-dependent translation in infected L cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 152-6

Skup D, Millward S

Abstract

The translational apparatus in cell-free extracts prepared from L cells infected with reovirus undergoes a time-dependent transition from cap dependence to cap independence. Extracts from uninfected L cells translate capped reovirus mRNA at high efficiency and synthesize the expected three size classes of reovirus polypeptides, and the translation is sensitive to m7G(5')ppp. This same extract translates uncapped mRNA at a much lower efficiency. In contrast, extracts from infected L cells translate uncapped reovirus mRNA at high efficiency and synthesize the correct three size classes of polypeptides, and the translation is not sensitive to inhibition by m7G(5')ppp. Infected cell extracts translate capped mRNA at reduced efficiency (a,proximately 25%), the translation is not sensitive to inhibition by m7G(5')ppp, and the correct three size classes of viral polypeptides are not synthesized. These observations may explain how reovirus takes over the host translational apparatus.

MeSH Terms
Animals Cell-Free System L Cells Mice Protein Biosynthesis RNA Caps/metabolism RNA, Viral/genetics,metabolism Reoviridae/genetics,metabolism Reoviridae Infections/metabolism Structure-Activity Relationship
Chemicals
RNA Caps RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skup D
Millward S
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Asynchronous synthesis of the complementary strands of the reovirus genome.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):505-8 PMID: 5277109
  3. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5 PMID: 1054852
  4. 5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.
    Nature. 1975 May 1;255(5503):33-7 PMID: 165427
  5. Methylation-dependent translation of viral messenger RNAs in vitro.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189-93 PMID: 165487
  6. m7G5'ppp5'GmptcpUp at the 5' terminus of reovirus messenger RNA.
    Nucleic Acids Res. 1975 Aug;2(8):1329-43 PMID: 1178520
  7. Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):19-23 PMID: 1061116
  8. Regulated transcription of the genomes of defective virions and temperature-sensitive mutants of reovirus.
    J Virol. 1976 Apr;18(1):7-19 PMID: 1255877
  9. Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis.
    J Virol. 1976 May;18(2):719-26 PMID: 178904
  10. Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3.
    Nature. 1976 May 27;261(5558):291-4 PMID: 944862
  11. Translational discrimination of 'capped' and 'non-capped' mRNAS: inhibition of a series of chemical analogs of m7GpppX.
    FEBS Lett. 1976 May 1;64(2):326-31 PMID: 179883
  12. Translation in vitro of vesicular stomatitis virus mRNA lacking 5'-terminal 7-methylguanosine.
    Nature. 1976 Jul 1;262(5563):32-7 PMID: 180425
  13. 7-Methyl-guanosine and efficiency of RNA translation.
    J Virol. 1976 Aug;19(2):637-42 PMID: 957483
  14. Translational control of protein synthesis.
    Annu Rev Biochem. 1976;45:39-72 PMID: 786155
  15. Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate.
    J Biol Chem. 1976 Sep 25;251(18):5657-62 PMID: 965382
  16. Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3448-52 PMID: 185613
  17. 5'-Capping structures of Artemia salina mRNA and the translational inhibition by cap analogs.
    Eur J Biochem. 1976 Dec;71(1):281-93 PMID: 1009952
  18. Sodium ions and the shut-off of host cell protein synthesis by picornaviruses.
    Nature. 1976 Dec 23-30;264(5588):807-9 PMID: 1012329
  19. Capping of eucaryotic mRNAs.
    Cell. 1976 Dec;9(4 PT 2):645-53 PMID: 1017010
  20. Relative importance of 7-methylguanosine in ribosome binding and translation of vesicular stomatitis virus mRNA in wheat germ and reticulocyte cell-free systems.
    J Biol Chem. 1977 Feb 25;252(4):1181-8 PMID: 190222
  21. 5'-Terminal structure and mRNA stability.
    Nature. 1977 Mar 17;266(5599):235-9 PMID: 557727
  22. Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.
    Cell. 1977 May;11(1):73-82 PMID: 872220
  23. Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8 PMID: 197518
  24. 5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3254-8 PMID: 269388
  25. A simple method for the preparation of [beta-32P]purine nucleoside triphosphase.
    Nucleic Acids Res. 1977 Oct;4(10):3341-55 PMID: 928062
  26. Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.
    Nucleic Acids Res. 1977 Oct;4(10):3581-7 PMID: 928069
  27. Membrane leakiness after viral infection and a new approach to the development of antiviral agents.
    Nature. 1978 Apr 20;272(5655):694-9 PMID: 205795
  28. Inhibition of translation by poliovirus: inactivation of a specific initiation factor.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732-6 PMID: 208073
  29. Effect of enzymatic "decapping" on protamine mRNA translation in wheat germ S-30.
    Biochem Biophys Res Commun. 1978 Nov 14;85(1):114-24 PMID: 743265
  30. Polypeptide components of virions, top component and cores of reovirus type 3.
    Virology. 1969 Dec;39(4):791-810 PMID: 4311639
  31. Studies on the intracellular synthesis of reovirus-specified proteins.
    Virology. 1970 Jul;41(3):501-18 PMID: 5463865
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.1.152
Published
1980-01-00
Pages
152-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348226
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.1.152 PMC Full Text View journal details
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