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PMID: 6245064 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of lambda specialized transducing bacteriophages carrying Klebsiella pneumoniae nif genes.

Journal of bacteriology ·Vol. 141 ·No. 3 ·1980-03-00 ·Pages 1264-71

MacNeil D, Howe MM, Brill WJ

Abstract

Seve lambda dnif specialized transducing bacteriophages were isolated from Escherichia coli strains containing plasmids carrying the his-nif region of Klebsiella pneumoniae. These phages collectively carry deoxyribonucleic acid for all of the genes in the nif regulon and adjacent deoxyribonucleic acid of K. pneumoniae. The phages were isolated by using Mu insertions in the nif region to direct the integration of lambda pMu phages in nif via formation of lambda pMu-Mu dilysogens which, upon induction, yielded lambda dnif phages. This procedure should be generally applicable for isolating lambda specialized transducing phages carrying genes from E. coli or other bacteria.

MeSH Terms
Bacteriophage lambda/genetics Bacteriophage mu/genetics DNA Transposable Elements Genes Genetic Techniques Klebsiella pneumoniae/genetics,metabolism Lysogeny Nitrogen Fixation Recombination, Genetic Transduction, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
MacNeil D
Howe M M
Brill W J
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.3.1264-1271.1980
Published
1980-03-00
Pages
1264-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293822
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.141.3.1264-1271.1980 PMC Full Text View journal details
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