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PMID: 6248723 Published · ppublish English Journal Article

IS4 is still found at its chromosomal site after transposition to galT.

Molecular & general genetics : MGG ·Vol. 178 ·No. 2 ·1980-00-00 ·Pages 281-4

Klaer R, Pfeifer D, Starlinger P

Abstract

IS4-DNA has been hybridized to separated DNA fragments of E. coli K12 strain M28 and to three mutants caused by transposition of IS4 to galT. The parental strain shows one band hybridizing to IS4 representing one copy of IS4 in the chromosome. The mutants have this copy retained and show in addition a second band corresponding to the IS4 copy in galT. The experiments support the hypothesis that transposition of IS4 is accompanied by replication of the element.

MeSH Terms
DNA Replication DNA Restriction Enzymes/metabolism DNA Transposable Elements DNA, Bacterial/genetics,isolation & purification Electrophoresis, Agar Gel Escherichia coli/genetics Mutation Nucleic Acid Hybridization
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klaer R
Pfeifer D
Starlinger P
References (23)
23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270473
Published
1980-00-00
Pages
281-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270473
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