Abstract
The 24.5-megadalton plasmid of Neisseria gonorrhoeae is required for transfer of R-factors and possibly chromosomal markers during conjugal matings between gonococcal strains. We constructed a physical map of one such plasmid, pLE2451, using EcoRI, BglII, and HincII site-specific restriction endonucleases. The patterns of deoxyribonucleic acid digestion obtained with this plasmid were identical to those obtained with three other plasmids of similar size.
MeSH Terms
Conjugation, Genetic
DNA Restriction Enzymes/pharmacology
DNA, Bacterial/analysis,genetics
Molecular Weight
Neisseria gonorrhoeae/analysis,genetics
Plasmids
Chemicals
DNA, Bacterial
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tenover F C
Mayer L W
Young F E
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14 references, click to expand
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