Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.
A serological trapping technique for detecting rotaviruses is described which involves coating electron microscope grids with protein A and specific rotavirus antiserum. The presence of a layer of antibodies on the grid increases the number of rotavirus particles that can be visualized. Thirty-five crude fecal extracts from infants suffering from diarrhea were examined by the serological trapping technique and by standard electron-microscopy. When the specimens were deposited on antibody-coated girds, 71% of them were found to contain virus particles, compared with 20% on standard uncoated grids. The method is simple and rapid and does away with the need to concentrate the specimens.
15 references, click to expand
-
Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.J Gen Virol. 1977 May;35(2):203-18 PMID: 406356
-
[Application of enzyme-linked immunosorbent assay (ELISA) to the detection of calf rotavirus and rotavirus antibodies (author's transl)].Ann Microbiol (Paris). 1977 May-Jun;128A(4):499-510 PMID: 199102
-
Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves.J Clin Microbiol. 1977 Nov;6(5):530-2 PMID: 200635
-
Rotaviruses.J Gen Virol. 1978 Jul;40(1):1-18 PMID: 80441
-
An immunoelectron microscopic investigation of oat sterile dwarf and related viruses.Virology. 1978 Oct 15;90(2):299-304 PMID: 726254
-
Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.J Clin Microbiol. 1979 Sep;10(3):317-21 PMID: 226564
-
Viral gastroenteritis.Prog Med Virol. 1979;25:1-36 PMID: 228348
-
A solid-phase system (SPACE) for the detection and quantification of rotavirus in faeces.J Gen Virol. 1979 Sep;44(3):615-23 PMID: 231086
-
"Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.J Immunol. 1966 Dec;97(6):822-7 PMID: 4163007
-
Quantitative assay for plant viruses using serologically specific electron microscopy.Virology. 1973 Dec;56(2):652-3 PMID: 4796553
-
Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.Science. 1974 Sep 20;185(4156):1049-53 PMID: 4605043
-
Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin.J Virol. 1975 Nov;16(5):1229-35 PMID: 171450
-
Comparison of Counterimmunoelectrophoresis and electron microscopy for laboratory diagnosis of human reovirus-like agent-associated infantile gastroenteritis.J Clin Microbiol. 1977 Feb;5(2):248-9 PMID: 403200
-
A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools.J Immunol. 1977 Apr;118(4):1275-9 PMID: 321688
-
Solid-phase radioimmunoassay for the detection of rotavirus.Infect Immun. 1977 May;16(2):439-44 PMID: 863510
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
