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PMID: 6253904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11.

Nucleic acids research ·Vol. 8 ·No. 16 ·1980-08-25 ·Pages 3591-601

Zakut R, Cohen J, Givol D

Abstract

Poly(A)-containing mRNA from mouse myeloma MPC11 was transcribed into cDNA which was cloned in the PstI site of the plasmid pBR322. The transformants were screened by hybridization with a cDNA fragment, derived from plasmid p gamma(11)7, corresponding to the 5' portion of the constant region of MPC11 heavy chain. Several positive transformants were found to contain various lengths of the variable region of the heavy chain. We describe the structure and sequence of one of these clones, pV(11)2, which contains cDNA corresponding to the entire variable region of MPC11 heavy chain and extends to codon 248 in the constant region. The protein sequence deduced from the DNA sequence indicates that the variable region of MPC11 heavy chain contains 121 amino acids and belongs to subgroup II of mouse heavy chains. Comparison of this sequence with other heavy chain sequences suggests a J (joining) segment of 16 residues which overlaps five residues of the third hypervariable region. The cDNA sequence shows that there is no discontinuity between the end of the variable region and the beginning of the constant region.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genetic Code Immunoglobulin Heavy Chains/biosynthesis Mice Molecular Weight Nucleic Acid Hybridization Plasmacytoma Poly A/biosynthesis RNA, Messenger/biosynthesis Transcription, Genetic
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains RNA, Messenger Poly A DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zakut R
Cohen J
Givol D
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.16.3591
Published
1980-08-25
Pages
3591-601
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324177
Subset
IM
Databases
GENBANK
J00484, K00698, L00050, L00051
External Links
PubMed source DOI: 10.1093/nar/8.16.3591 PMC Full Text View journal details
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