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PMID: 6253925 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The organization of the ribosomal RNA genes in the fungus Mucor racemosus.

Nucleic acids research ·Vol. 8 ·No. 4 ·1980-02-25 ·Pages 793-804

Cihlar RL, Sypherd PS

Abstract

The rDNA of Mucor racemosus is contained on a 6.4 megadalton repeat unit. Two Bam H-1 restriction fragments that encompass the entire rDNA repeat, as well as two Hind III restriction fragments that lie within the region, have been cloned and analyzed. The rDNA unit has been defined with respect to eight restriction endonucleases and the position of the sequences encoding the 25S, 18S, and 5.8S rRNA species have been localized. In addition, the 55 RNA encoding sequence was found to reside within the basic repeat unit. The results indicate the organization of the rDNA of Mucor more closely resembles the arrangement observed in yeast than that observed in other eukaryotic organisms.

MeSH Terms
Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Genes Mucor/genetics Nucleic Acid Hybridization RNA, Ribosomal/genetics
Chemicals
DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cihlar R L
Sypherd P S
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1980-02-25
Pages
793-804
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327311
Subset
IM
Grants
NIGMS NIH HHS · GM 26690 · United States
External Links
PubMed source PMC Full Text View journal details
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