Construction of a composite tRNA gene by anticodon loop transplant.
By using sites for the restriction nuclease Hpa II, the information for the anticodon stem and loop of an altered Su+2 amber suppressor tRNA (a mutant of tRNAGln) has been transplanted to a specially prepared tRNATrp gene, which lacks it homologous anticodon stem and loop sequence. The resulting tRNA gene was cloned under lac operator-promoter control. The result is a functional, hybrid, amber-suppressor tRNA that can exhibit a moderately high efficiency in translation. It appears less efficient, however, than Su+7 tRNA, the amber suppressor that results from a direct anticodon mutation in tRNATrp. As judged by its suppressor spectrum, which is almost identical to the spectra of Su+2, and Su+7, the recomposed tRNA inserts glutamine at amber sites. This experiment is the prototype of a series of construction that examine the role of the nucleotides in the anticodon region.
29 references, click to expand
-
Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene.J Mol Biol. 1965 Dec;14(2):522-7 PMID: 4286570
-
Amber suppressors: efficiency of chain propagation and suppressor specific amino acids.J Mol Biol. 1965 Dec;14(2):528-33 PMID: 5880864
-
Nonsense codons and polarity in the tryptophan operon.J Mol Biol. 1966 Nov 14;21(2):313-34 PMID: 5339605
-
Mutants that make more lac repressor.Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259-64 PMID: 4870861
-
Recessive lethal nonsense suppressor in Escherichia coli which inserts glutamine.Nature. 1969 Sep 27;223(5213):1340-2 PMID: 4897374
-
Ribosomal mutations affecting efficiency of amber suppression.J Mol Biol. 1970 Feb 14;47(3):517-30 PMID: 4907270
-
Release factors mediating termination of complete proteins.Nature. 1970 Jun 13;226(5250):1029-33 PMID: 4911223
-
Effect of Sul gene dosage on the production of suppressed alkaline phosphatase in Escherichia coli K12.J Mol Biol. 1970 Apr 14;49(1):241-4 PMID: 4915860
-
Tryptophan transfer RNA as the UGA suppressor.J Mol Biol. 1971 Jun 14;58(2):439-58 PMID: 4933412
-
Oligonucleotide sequences of RNase T 1 and pancreatic RNase digests of E. coli aspartic acid tRNA.Biochem Biophys Res Commun. 1972 Dec 18;49(6):1605-9 PMID: 4565380
-
The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585-9 PMID: 4587256
-
A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG.J Mol Biol. 1974 Jun 25;86(2):245-60 PMID: 4606150
-
Mutational alterations of tryptophan-specific transfer RNA that generate translation suppressors of the UAA, UAG and UGA nonsense codons.J Mol Biol. 1974 Jun 25;86(2):233-43 PMID: 4606830
-
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.Cell. 1974 Dec;3(4):315-25 PMID: 4216403
-
Suppressor su+7 inserts tryptophan in addition to glutamine.J Mol Biol. 1976 Jul 5;104(3):729-34 PMID: 781298
-
A new method for sequencing DNA.Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
-
Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends.J Biol Chem. 1977 Jun 10;252(11):3987-94 PMID: 863910
-
Some rRNA operons in E. coli have tRNA genes at their distal ends.Cell. 1978 Feb;13(2):335-44 PMID: 342117
-
Control of transcription termination.Annu Rev Biochem. 1978;47:967-96 PMID: 354508
-
The purification and sequence of a temperature-sensitive tryptophan tRNA.J Biol Chem. 1979 Jun 25;254(12):5562-6 PMID: 109437
-
The structure of the phi 80d3 ilv+ Su+7 transducing phage and the origin of its Su+7 tRNA-gene containing fragment.Mol Gen Genet. 1979 Mar 5;170(3):283-9 PMID: 287851
-
Isolation and properties of a plasmid which expresses the E. coli Su+7 amber suppressor tRNA gene.Mol Gen Genet. 1979 Mar 5;170(3):291-8 PMID: 379544
-
The structure of the DNA containing the E. coli tRNATry gene.Mol Gen Genet. 1979 Mar 5;170(3):299-308 PMID: 379545
-
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
-
Transcription termination in the Escherichia coli ribosomal RNA operon rrnC.J Biol Chem. 1979 Dec 25;254(24):12725-31 PMID: 115888
-
The structure and aminoacylation of a temperature-sensitive tRNATrp (Escherichia coli).J Biol Chem. 1980 Feb 10;255(3):1128-37 PMID: 6766136
-
Identification of transfer RNA suppressors in Escherichia coli. I. Amber suppressor su+2, an anticodon mutant of tRNA2Gln.J Mol Biol. 1979 Aug 25;132(4):649-62 PMID: 160949
-
Dual specificity of su+ 7 tRNA. Evidence for translational discrimination.J Mol Biol. 1980 Jun 5;139(4):705-20 PMID: 6997500
-
The properties of altered proteins from mutants bearing one or two lesions in the same gene.Proc Natl Acad Sci U S A. 1961 Apr 15;47:551-66 PMID: 13765795
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
