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PMID: 6255426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Accessibility of expressed and non-expressed genes to a restriction nuclease.

Nucleic acids research ·Vol. 8 ·No. 20 ·1980-10-24 ·Pages 4621-38

Pfeiffer W, Zachau HG

Abstract

Nuclei of mouse liver and an immunoglobulin producing myeloma were digested with HaeIII or its isoschizomer BspRI. The DNA fragment patterns after electrophoresis, blotting, and hybridization were very similar for the two types of nuclei when a probe for the non-expressed beta-globin gene was used. The constant (C) region of the kappa light chain gene, on the other hand, was more accessible to the nuclease in myeloma than on liver nuclei. In myeloma nuclei the BspRI sites were about equally sensitive, in fact the pattern resembled a partial digestion pattern of free DNA. In contrast, in liver nuclei some sites were much more protected than others. This is interpreted by assuming that this single copy non-expressed gene region is covered by nucleosomes which are preferentially located on certain DNA sequences. Restriction nuclease digestion of nuclei seems to be a promising method for the analysis of genes in their expressed and non-expressed states.

MeSH Terms

Animals Base Sequence Cell Line Cell Nucleus/analysis DNA/analysis DNA Restriction Enzymes Genes Immunoglobulins/biosynthesis Liver/analysis Mice Neoplasms, Experimental/analysis Plasmacytoma/analysis Plasmids

Chemicals

Immunoglobulins DNA DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pfeiffer W

Zachau H G

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.20.4621

Published

1980-10-24

Pages

4621-38

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324375

Subset

External Links

PubMed source DOI: 10.1093/nar/8.20.4621 PMC Full Text View journal details

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Accessibility of expressed and non-expressed genes to a restriction nuclease.

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