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PMID: 6253943 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.

Nucleic acids research ·Vol. 8 ·No. 8 ·1980-04-25 ·Pages 1693-707

Steinmetz M, Zachau HG

Abstract

The organization of immunoglobulin gene segments coding for kappa light chains has been studied in uncloned and cloned DNA from mouse liver and a mouse myeloma. It is known that the C (constant, ref. 2) gene segment is present in the tumor DNA on two EcoRI fragments of 14 and 20 kb and in liver DNA on a 15 kb fragment. The 14 kb myeloma and the 15 kb liver fragment have been cloned previously. Here we report on the cloning of the 20 kb myeloma fragment and present detailed restriction maps covering about 22 kb of DNA surrounding the C gene segment in liver and tumor DNA. The region on the 20 kb fragment has been localized where a DNA rearrangement had occurred. The presence of two rearranged kappa light chain genes in one tumor is discussed in regard to the molecular basis of allelic exclusion.

MeSH Terms
Animals Cell Line DNA/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/metabolism Genes Immunoglobulin Light Chains/biosynthesis Liver/metabolism Mice Molecular Weight Plasmacytoma Plasmids
Chemicals
DNA, Recombinant Immunoglobulin Light Chains DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Steinmetz M
Zachau H G
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.8.1693
Published
1980-04-25
Pages
1693-707
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324029
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.8.1693 PMC Full Text View journal details
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