Home LiteratureArticle Details
PMID: 6253945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of V region fragments from mouse liver DNA and localization of repetitive DNA sequences in the vicinity of immunoglobulin gene segments.

Nucleic acids research ·Vol. 8 ·No. 8 ·1980-04-25 ·Pages 1721-9

Steinmetz M, Höchtl J, Schnell H, Gebhard W, Zachau HG

Abstract

Two different kappa light chain genes have previously been isolated from one mouse myeloma. The V (variable, abbreviations in ref. 2) gene segments of the two genes were now used to identify their germline counterparts in EcoRI digests of mouse liver DNA. In addition two sets of related V gene segments were found which hybridize with either of the two DNA probes. Five of the V region fragments of one set were cloned in a lambda phage vector and partially characterized by restriction mapping and Southern blot hybridization. Repetitive DNA sequences were found on each of the five fragments as well as on other cloned immunoglobulin gene containing fragments. Cross-hybridization between some but not all of the regions containing repetitive DNA sequences was observed.

MeSH Terms
Animals Binding Sites, Antibody Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Immunoglobulin Variable Region Liver/immunology Mice Neoplasms, Experimental/immunology Nucleic Acid Hybridization Plasmacytoma/immunology Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Steinmetz M
Höchtl J
Schnell H
Gebhard W
Zachau H G
References (18)
18 references, click to expand
  1. Organization of highly repeated sequences in mouse main-band DNA.
    J Mol Biol. 1976 Jan 25;100(3):227-56 PMID: 768484
  2. Characterization of distinct segments in mouse satellite DNA by restriction nucleases.
    Eur J Biochem. 1977 Mar 1;73(2):383-92 PMID: 321221
  3. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
    Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
  4. Antibody diversity.
    Science. 1978 Oct 6;202(4363):11-7 PMID: 99815
  5. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  6. Comparison of cloned mouse alpha- and beta-globin genes: conservation of intervening sequence locations and extragenic homology.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6187-91 PMID: 282635
  7. DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4709-13 PMID: 105355
  8. Long double-stranded sequences (dsRNA-B) of nuclear pre-mRNA consist of a few highly abundant classes of sequences: evidence from DNA cloning experiments.
    Nucleic Acids Res. 1979 Feb;6(2):697-713 PMID: 370792
  9. A kappa-immunoglobulin gene is formed by site-specific recombination without further somatic mutation.
    Nature. 1979 Aug 2;280(5721):370-5 PMID: 111146
  10. Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.
    Nucleic Acids Res. 1979 Jul 25;6(10):3213-29 PMID: 113775
  11. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  12. RNA polymerase III transcriptional units are interspersed among human non-alpha-globin genes.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5095-9 PMID: 388428
  13. Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4627-31 PMID: 116236
  14. Rearrangement of immunoglobulin genes.
    Biochemistry. 1979 Oct 30;18(22):5013-21 PMID: 508729
  15. Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes.
    Cell. 1980 Jan;19(1):37-51 PMID: 6766810
  16. Organization and sequence studies of the 17-piece chicken conalbumin gene.
    Nature. 1979 Dec 6;282(5739):567-74 PMID: 551292
  17. Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.
    Nucleic Acids Res. 1980 Apr 25;8(8):1693-707 PMID: 6253943
  18. A rearranged DNA sequence possibly related to the translocation of immunoglobulin gene segments.
    Nucleic Acids Res. 1980 Apr 25;8(8):1709-20 PMID: 6253944
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.8.1721
Published
1980-04-25
Pages
1721-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324031
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.8.1721 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2