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PMID: 6255467 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 5822-6

Simpson RU, DeLuca HF

Abstract

Isolated rat epidermis possesses a cytosolic 3.5 S receptor-like protein for 1,25-dihydroxyvitamin D3. This 3.5S binder has a high affinity (Kd = 1.4 X 10(-10) M) for 1,25-dihydroxyvitamin D3 and is present in low concentrations (31 fmol of binding sites per mg of cytosol protein). Analog competition for receptor binding revealed the following potency order: 1,25-dihydroxyvitamin D3 > 25-hydroxyvitamin D3 > 1 alpha-hydroxyvitamin D3 > 24 (R),25-dihydroxyvitamin D3 > vitamin D3. The receptor has a molecular weight of 60,000, has affinity for DNA-cellulose, and aggregates in the presence of low potassium concentrations. The 1,25-dihydroxyvitamin D3 binder is stabilized by sodium molybdate (10 mM). Addition of phenylmethylsulfonyl fluoride (3 mM) was found to yield more reproducible receptor preparations. The presence of a 1,25-dihydroxyvitamin D3 binding macromolecule with properties similar to those of the bone and intestinal receptor suggests that skin is an additional target organ for this hormone.

MeSH Terms
Animals Calcitriol Cytosol/metabolism Dihydroxycholecalciferols/metabolism Epidermis/metabolism Hydroxycholecalciferols/metabolism Ligands Molecular Weight Protein Binding Rats Receptors, Calcitriol Receptors, Steroid/metabolism Skin/metabolism Structure-Activity Relationship
Chemicals
Dihydroxycholecalciferols Hydroxycholecalciferols Ligands Receptors, Calcitriol Receptors, Steroid Calcitriol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simpson R U
DeLuca H F
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.10.5822
Published
1980-10-00
Pages
5822-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350163
Subset
IM
Grants
NIADDK NIH HHS · AM-06369-01 · United States
NIADDK NIH HHS · AM-14881 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.10.5822 PMC Full Text View journal details
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