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PMID: 6260673 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.

Infection and immunity ·Vol. 31 ·No. 2 ·1981-02-00 ·Pages 660-7

Norrild B, Pedersen B, Roizman B

Abstract

In this paper we report that viral polypeptides from herpes simplex virus 1 (HSV-1) and 2 (HSV-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. The polyvalent hyperimmune sera against HSV-1 reacted with 17 HSV-1 polypeptide bands and 8 HSV-2 polypeptide bands. Concordantly, polyvalent sera against HSV-2 reacted with at least 16 HSV-2 polypeptide bands and 8 HSV-1 polypeptide bands. The antisera prepared against the specific antigens reacted with a smaller number of polypeptide bands. Preimmune sera and immune sera did not react with electrophoretically separated polypeptides from infected and uninfected cells, respectively. The immune localization of separated antigens test provides a powerful technique for identification of immunogenic viral polypeptides, especially those which are normally insoluble and therefore unavailable for immunological reactivity in immune precipitation tests.

MeSH Terms
Animals Antigen-Antibody Reactions Antigens, Viral Electrophoresis, Polyacrylamide Gel/methods Methylcellulose/analogs & derivatives Simplexvirus/immunology Viral Proteins/immunology
Chemicals
Antigens, Viral Viral Proteins diazobenzyloxymethyl cellulose Methylcellulose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Norrild B
Pedersen B
Roizman B
References (28)
28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.31.2.660-667.1981
Published
1981-02-00
Pages
660-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351360
Subset
IM
Grants
NCI NIH HHS · CA 19264-05 · United States
NCI NIH HHS · CA-08494-15 · United States
External Links
PubMed source DOI: 10.1128/iai.31.2.660-667.1981 PMC Full Text View journal details
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