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PMID: 6261013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Properties of simian virus 40 small t antigen overproduced in bacteria.

Journal of virology ·Vol. 37 ·No. 2 ·1981-02-00 ·Pages 683-97

Thummel CS, Burgess TL, Tjian R

Abstract

We constructed a series of bacterial plasmids which contained the Escherichia coli lac promoter fused to a simian virus 40 restriction fragment coding for small t antigen. These plasmids expressed different levels of intact viral protein depending on the length of the constructed ribosome binding site. Small t antigen synthesized by the most efficient producer, HP1, constituted 0.5 to 1% of the total cellular protein. On the basis of extensive characterization by immunoprecipitation, gel electrophoresis, isoelectric focusing, tryptic fingerprint analysis, and chromatographic properties, this plasmid-encoded protein was virtually identical to authentic simian virus 40 small t antigen. Partial purification of the HP1-encoded and authentic small t antigens revealed the presence of both monomeric and multimeric forms.

MeSH Terms
Antigens, Neoplasm/biosynthesis,immunology,isolation & purification Antigens, Viral/biosynthesis,immunology,isolation & purification Antigens, Viral, Tumor Base Sequence Chromatography, Gel Cloning, Molecular Escherichia coli/genetics Operon Plasmids Ribosomes/metabolism Simian virus 40/immunology
Chemicals
Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thummel C S
Burgess T L
Tjian R
References (52)
52 references, click to expand
  1. Simian virus 40 small t antigen is not required for the maintenance of transformation but may act as a promoter (cocarcinogen) during establishment of transformation in resting rat cells.
    J Virol. 1979 Dec;32(3):979-88 PMID: 229274
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  5. Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells.
    J Virol. 1980 Mar;33(3):1182-91 PMID: 6245268
  6. Oncogenicity of simian virus 40 deletion mutants that induce altered 17-kilodalton t-proteins.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4299-302 PMID: 228294
  7. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):457-61 PMID: 191809
  8. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  9. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.
    Nature. 1979 Oct 18;281(5732):544-8 PMID: 386136
  10. Autoregulation of simian virus 40 gene A by T antigen.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3083-7 PMID: 184459
  11. A general method for maximizing the expression of a cloned gene.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):760-4 PMID: 370836
  12. Chicken ovalbumin is synthesized and secreted by Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5936-40 PMID: 104296
  13. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  14. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells.
    Cell. 1979 Jul;17(3):635-43 PMID: 225037
  15. End group labelling of RNA and double stranded DNA by phosphate exchange catalyzed by bacteriophage T4 induced polynucleotide kinase.
    Biochem Biophys Res Commun. 1975 Oct 6;66(3):962-9 PMID: 170940
  16. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  17. Human growth hormone: complementary DNA cloning and expression in bacteria.
    Science. 1979 Aug 10;205(4406):602-7 PMID: 377496
  18. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  19. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  20. Anchorage independence: analysis of factors affecting the growth and colony formation of wild-type and dl 54/59 mutant SV40-transformed lines.
    Virology. 1979 Dec;99(2):302-11 PMID: 229626
  21. Contacts between Escherichia coli RNA polymerase and a lac operon promoter.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5314-8 PMID: 364474
  22. Variable defectiveness for lytic growth of the dl 54/59 mutants of simian virus 40.
    J Virol. 1980 Mar;33(3):1208-10 PMID: 6245271
  23. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  24. Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells.
    Cell. 1978 May;14(1):79-88 PMID: 208777
  25. Maximizing gene expression on a plasmid using recombination in vitro.
    Cell. 1978 Jan;13(1):65-71 PMID: 340049
  26. Large and small tumor antigens from simian virus 40 have identical amino termini mapping at 0.65 map units.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2165-9 PMID: 209456
  27. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.
    Proc Natl Acad Sci U S A. 1963 Dec;50:1148-56 PMID: 14096190
  28. New region of the simian virus 40 genome required for efficient viral transformation.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2473-7 PMID: 209467
  29. Effect of alkylation on the physical properties of simian virus 40 T-antigen species.
    J Virol. 1979 Feb;29(2):587-96 PMID: 219248
  30. An immunochemical investigation of SV40 T-antigens. 2. Quantitation of antigens and antibody activities.
    Virology. 1980 Jan 30;100(2):314-25 PMID: 6153245
  31. Structural relationship between the 100,000- and 17,000- molecular-weight T antigens of simian virus 40 (SV40) as deduced by comparison with the SV40-specific proteins coded by the nondefective adenovirus type 2-SV40 hybrid viruses.
    J Virol. 1979 Jan;29(1):390-4 PMID: 219231
  32. The vertebrate eye lens.
    Science. 1977 Jul 8;197(4299):127-38 PMID: 877544
  33. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  34. Viable deletion mutants in the simian virus 40 early region.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4455-9 PMID: 212752
  35. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  36. "Rescued" SV40: increased transforming efficiency in mouse and human cells.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1031-7 PMID: 4307871
  37. Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid.
    Nature. 1978 Oct 12;275(5680):505-10 PMID: 80751
  38. Isolation of the lac repressor.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8 PMID: 16591435
  39. Function of simian virus 40 gene A in transforming infection.
    J Virol. 1975 Mar;15(3):613-8 PMID: 163375
  40. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  41. Relationship of replication and transcription of Simian Virus 40 DNA.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):1927-30 PMID: 4352963
  42. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
  43. Synthesis of simian virus 40 t antigen in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5596-600 PMID: 230483
  44. Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.
    Nucleic Acids Res. 1975 Sep;2(9):1459-92 PMID: 1178526
  45. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  46. Organization and expression of early genes of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
  47. Isolation and characterization of T antigen-negative revertants from a line of transformed rat cells containing one copy of the SV40 genome.
    Cell. 1978 Jan;13(1):19-32 PMID: 202400
  48. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  49. Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.
    J Virol. 1978 Oct;28(1):140-53 PMID: 212599
  50. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  51. Mutants that make more lac repressor.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259-64 PMID: 4870861
  52. The structure and expression of two defective adenovirus 2/simian virus 40 hybrids.
    J Mol Biol. 1978 Apr 5;120(2):209-47 PMID: 205653
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.2.683-697.1981
Published
1981-02-00
Pages
683-97
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171056
Subset
IM
Grants
NCI NIH HHS · CA25417 · United States
NIEHS NIH HHS · ES01896 · United States
External Links
PubMed source DOI: 10.1128/JVI.37.2.683-697.1981 PMC Full Text View journal details
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