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PMID: 6262248 Published · ppublish English Journal Article

Role of antibody to leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis.

Infection and immunity ·Vol. 31 ·No. 3 ·1981-03-00 ·Pages 1223-31

Sato Y, Izumiya K, Sato H, Cowell JL, Manclark CR

Abstract

Gamma globulins were obtained from rabbits hyperimmunized with the filamentous hemagglutinin (FHA) or the leukocytosis-promoting factor hemagglutinin (LPF.HA) of Bordetella pertussis. Double immunodiffusion analyses showed the antibodies to be highly specific for the homologous antigen. Intraperitoneal injection of anti-FHA or anti-LPF.HA into 10-day-old ICR mice 30 min before aerosol challenge with B. pertussis protected the mice from disease as evidenced by survival and a normal rate of gain in body weight. Both anti-LPF.HA and anti-FHA enhanced the clearance of B. pertussis from the lung and prevented leukocytosis which normally occurs after aerosol infection of mice with B. pertussis. Anti-LPF.HA, but not anti-FHA, neutralized the leukocytosis-promoting and histamine-sensitizing activities of LPF.HA when incubated with the purified protein in vitro. Anti-FHA, but not anti-LPF.HA, prevented the attachment of B. pertussis to mammalian cells (HeLa and Vero) in culture.

MeSH Terms
Animals Antibodies, Bacterial Antigens, Bacterial/analysis Bordetella pertussis/immunology Disease Models, Animal Hemagglutinins/immunology Immunization, Passive Interleukin-2/immunology Lymphokines/immunology Mice Pertussis Toxin Pertussis Vaccine/analysis Rabbits Virulence Factors, Bordetella
Chemicals
Antibodies, Bacterial Antigens, Bacterial Hemagglutinins Interleukin-2 Lymphokines Pertussis Vaccine Virulence Factors, Bordetella Pertussis Toxin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sato Y
Izumiya K
Sato H
Cowell J L
Manclark C R
References (19)
19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.31.3.1223-1231.1981
Published
1981-03-00
Pages
1223-31
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351446
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.31.3.1223-1231.1981 PMC Full Text View journal details
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