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PMID: 6262368 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization.

Journal of clinical microbiology ·Vol. 12 ·No. 2 ·1980-08-00 ·Pages 226-34

Brautigam AR, Richman DD, Oxman MN

Abstract

A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled deoxyribonucleic acid from infected cultures and tritium-labeled virus deoxyribonucleic acid, and it can be completed within a day using a single roller-tube culture of the clinical isolated. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thirty-nine coded clinical isolates were all correctly typed by this method.

MeSH Terms
DNA, Viral/analysis Herpes Simplex/diagnosis Humans Methods Neutralization Tests Nucleic Acid Conformation Nucleic Acid Hybridization Simplexvirus/classification Sonication
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brautigam A R
Richman D D
Oxman M N
References (38)
38 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.12.2.226-234.1980
Published
1980-08-00
Pages
226-34
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC273559
Subset
IM
Grants
NIAID NIH HHS · R01-AI-13872 · United States
External Links
PubMed source DOI: 10.1128/jcm.12.2.226-234.1980 PMC Full Text View journal details
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