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PMID: 6262811 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1527-31

O'Hare K, Benoist C, Breathnach R

Abstract

A recombinant plasmid has been constructed for the expression of inserted DNA sequences coding for polypeptide chains using the simian virus 40 early promoter and splicing and polyadenylylation signals from the rabbit beta-globin gene. The coding regions for two prokaryotic methotrexate-resistant dihydrofolate reductases were introduced into the expression vector. When mouse fibroblasts were exposed to these recombinant plasmids, it was possible to select methotrexate-resistant clones that had integrated the plasmids and produced a chimeric RNA coding for the prokaryotic enzyme.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Drug Resistance Genes Globins/genetics L Cells/metabolism Methotrexate/pharmacology Mice Plasmids Rabbits Tetrahydrofolate Dehydrogenase/genetics Transcription, Genetic Transformation, Genetic
Chemicals
DNA, Recombinant Globins Tetrahydrofolate Dehydrogenase DNA Restriction Enzymes Methotrexate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
O'Hare K
Benoist C
Breathnach R
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1527
Published
1981-03-00
Pages
1527-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319164
Subset
IM
Databases
GENBANK
K01478, K01479
External Links
PubMed source DOI: 10.1073/pnas.78.3.1527 PMC Full Text View journal details
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