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PMID: 6264445 Published · ppublish English Journal Article

Linkage of the four gamma subclass heavy chain genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 1 ·1981-01-00 ·Pages 474-8

Roeder W, Maki R, Traunecker A, Tonegawa S

Abstract

The genes for the heavy-chain constant regions of the four gamma subclass immunoglobulins were identified in a set of overlapping mouse DNA fragments representing about 100 kilobase pairs (kb) of the mouse genome that was cloned from bacteriophage lambda libraries of BALB/c mouse embryo DNA. R-loop mapping studies show that the genes are located 5'-C gamma 3-34 kb-C gamma 1-21 kb-C gamma 2b-15 kb-C gamma 2a-3' and lie in the same transcriptional orientation. Two DNA segments, one of 19 kb and another of 15 kb, that surround the C gamma 2b and C gamma 2a genes, respectively, show considerable homology and implicate a tandem duplication mechanism in the evolution of this gene cluster.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes Embryo, Mammalian Escherichia coli/genetics Genetic Linkage Immunoglobulin Heavy Chains/genetics Immunoglobulin gamma-Chains/genetics Mice/genetics Mice, Inbred BALB C Nucleic Acid Hybridization
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin gamma-Chains DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Roeder W
Maki R
Traunecker A
Tonegawa S
References (33)
33 references, click to expand
  1. Immunoglobulins as cell receptors.
    Ann N Y Acad Sci. 1971 Dec 31;190:420-31 PMID: 4944965
  2. Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA.
    Nucleic Acids Res. 1980 Jul 25;8(14):3143-55 PMID: 6777755
  3. Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3628-32 PMID: 824647
  4. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  6. Studies of generation of B-cell diversity in mouse, man, and chicken.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:139-45 PMID: 408085
  7. Synthesis of multiple immunoglobulin classes by single lymphocytes.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:175-83 PMID: 408086
  8. Cloning of an immunoglobulin variable region gene from mouse embryo.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3518-22 PMID: 410021
  9. Organization of immunoglobulin heavy chain genes and allelic deletion model.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2140-4 PMID: 97665
  10. Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma gamma1 chain.
    J Biol Chem. 1978 Sep 10;253(17):6068-75 PMID: 98524
  11. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  12. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  13. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  14. DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4709-13 PMID: 105355
  15. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  16. The ovalbumin gene region: common features in the organisation of three genes expressed in chicken oviduct under hormonal control.
    Nature. 1979 May 10;279(5709):125-32 PMID: 440416
  17. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  18. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  19. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  20. The role of gene deletion in the immunoglobulin heavy chain switch.
    Nature. 1980 Jan 24;283(5745):351-6 PMID: 6766206
  21. An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
    Nature. 1980 Feb 21;283(5749):733-9 PMID: 6766532
  22. Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes.
    Cell. 1980 Jan;19(1):37-51 PMID: 6766810
  23. Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):919-23 PMID: 6767246
  24. Rearrangement of immunoglobulin heavy chain genes during B-lymphocyte development as revealed by studies of mouse plasmacytoma cells.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1422-6 PMID: 6769113
  25. Exon shuffling generates an immunoglobulin heavy chain gene.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2138-42 PMID: 6769120
  26. Nucleotide sequence divergence of mouse immunoglobulin gamma 1 and gamma 2b chain genes and the hypothesis of intervening sequence-mediated domain transfer.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2143-7 PMID: 6769121
  27. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  28. Deletions in the constant region locus can account for switches in immunoglobulin heavy chain expression.
    Nature. 1980 Jun 12;285(5765):450-6 PMID: 6772957
  29. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  30. Structure and in vitro transcription of human globin genes.
    Science. 1980 Sep 19;209(4463):1329-36 PMID: 6158093
  31. Mapping of heavy chain genes for mouse immunoglobulins M and D.
    Science. 1980 Sep 19;209(4463):1348-53 PMID: 6774414
  32. DNA sequences mediating class switching in alpha-immunoglobulins.
    Science. 1980 Sep 19;209(4463):1360-5 PMID: 6774415
  33. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.1.474
Published
1981-01-00
Pages
474-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319076
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.1.474 PMC Full Text View journal details
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