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PMID: 6265916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 2843-7

Umeda PK, Sinha AM, Jakovcic S, Merten S, Hsu HJ, Subramanian KN, Zak R, Rabinowitz M

Abstract

Recombinant DNA clones containing sequences for two different types of myosin heavy chain (HC) genes from chicken embryonic skeletal muscle were constructed and analyzed. Specificity of the clones for myosin HC was demonstrated by hybrid-arrested translation, by hybridization to a 7.0-kb mRNA, and by comparison of DNA sequences with known amino acid sequences of rabbit skeletal muscle myosin HC. Restriction enzyme and electron-microscopic heteroduplex analysis showed the presence of two distinct but homologous cDNA sequences. Hybrid melting curves indicated that both types of sequences represent fast myosin HC sequences.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chick Embryo Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genetic Code Microscopy, Electron Muscles/metabolism Myosins/genetics Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Messenger DNA Restriction Enzymes Myosins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Umeda P K
Sinha A M
Jakovcic S
Merten S
Hsu H J
Subramanian K N
Zak R
Rabinowitz M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.2843
Published
1981-05-00
Pages
2843-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319454
Subset
IM
Grants
NHLBI NIH HHS · HL09172 · United States
NHLBI NIH HHS · HL20592 · United States
Databases
GENBANK
J00892
External Links
PubMed source DOI: 10.1073/pnas.78.5.2843 PMC Full Text View journal details
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