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PMID: 6265922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A RNA helix-destabilizing protein is a major component of Artemia salina nuclear ribonucleoproteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 2888-92

Thomas JO, Raziuddin, Sobota A, Boublik M, Szer W

Abstract

A major component of 30S heterogeneous nuclear ribonucleoprotein (hnRNP) particles from Artemia salina is HD40, a protein that has been characterized as a RNA helix-destabilizing protein [Marvil, D. K., Nowak, L. & Szer, W. (1980) J. Biol. Chem. 255, 6466-6472; Nowak, L., Marvil, D. K., Thomas, J. O., Boublik, M. & Szer, W. (1980) J. Biol. Chem. 255, 6473-6478]. HD40 binds to and disrupts the secondary structure of nuclear RNA fragments isolated from 30S hnRNP with a stoichiometry of one protein per 10-12 nucleotides. The addition of HD40 in excess of this ratio results in the formation of bead-like HD4-nuclear RNA complexes that are similar in properties and appearance to native 30S hnRNP particles. The heterogeneous nuclear RNA (hnRNA) in the HD40-hnRNA complexes is unstacked and unfolded to about the same extent as the RNA in the native 30S hnRNP particles. HD40 is strikingly similar in molecular weight (40,000) and amino acid composition (no cysteine, high glycine, presence of dimethylarginine, and blocked NH2 terminus) to eukaryotic hnRNP proteins isolated from many cell types. HD40 can be separated into three isoelectric species with basic pIs, which appears to be posttranslational modifications of a single polypeptide chain.

MeSH Terms
Animals Artemia/enzymology Cell Nucleus/enzymology Circular Dichroism DNA Helicases/isolation & purification DNA-Binding Proteins Microscopy, Electron Nucleoproteins/isolation & purification Protein Conformation Ribonucleoproteins/isolation & purification Viral Proteins
Chemicals
DNA-Binding Proteins Nucleoproteins Ribonucleoproteins Viral Proteins gp32 protein, Enterobacteria phage T4 helix-destabilizing proteins DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Thomas J O
Raziuddin
Sobota A
Boublik M
Szer W
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.2888
Published
1981-05-00
Pages
2888-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319464
Subset
IM
Grants
NCI NIH HHS · CA 16239 · United States
NIGMS NIH HHS · GM 23705 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.5.2888 PMC Full Text View journal details
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