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PMID: 6268847 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of the avian myelocytomatosis virus genome and recovery of infectious virus by transfection of chicken cells.

Journal of virology ·Vol. 39 ·No. 2 ·1981-08-00 ·Pages 625-31

Vennström B, Moscovici C, Goodman HM, Bishop JM

Abstract

The avian retrovirus myelocytomatosis virus 19 (MCV) possesses an interesting diversity of oncogenic potentials, but the virus has proven difficult to study because of its inability to replicate without the assistance of a helper virus. We have therefore isolated and amplified the genome of MCV by molecular cloning in a procaryotic vector. The topography of the cloned DNA was explored by the use of restriction endonucleases and radioactive complementary DNAs representing specific domains in avian retrovirus genomes. The cloned DNA appeared to be an authentic representation of the MCV genome: the size and genetic topography of the DNA were comparable to those of MCV, and transfection of the cloned DNA into chicken cells (in company with the DNA of a suitable helper virus) gave rise to virus with the genome and transforming potentials of MCV. The availability of cloned MCV DNA should facilitate a variety of genetic and biochemical manipulations directed at elucidating the mechanism of oncogenesis by MCV.

MeSH Terms
Animals Cell Transformation, Viral Cells, Cultured Chick Embryo Cloning, Molecular DNA Restriction Enzymes DNA, Viral Genes, Viral Retroviridae/genetics,physiology Transfection
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vennström B
Moscovici C
Goodman H M
Bishop J M
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.39.2.625-631.1981
Published
1981-08-00
Pages
625-31
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171372
Subset
IM
Grants
NCI NIH HHS · 1T32-CA-09043 · United States
NCI NIH HHS · CA-12705 · United States
NCI NIH HHS · CA-19287 · United States
External Links
PubMed source DOI: 10.1128/JVI.39.2.625-631.1981 PMC Full Text View journal details
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