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PMID: 6270352 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lack of AKR ecotropic provirus amplification in AKR leukemic thymuses.

Journal of virology ·Vol. 39 ·No. 3 ·1981-09-00 ·Pages 808-15

Yoshimura FK, Breda M

Abstract

A DNA fragment from the 3' region of a molecularly cloned AKR ecotropic provirus was identified to be specific for the AKR ecotropic murine leukemia virus (MuLV). This selected DNA fragment was used to analyze the integrated MuLV proviruses in normal and leukemic tissue DNAs of AKR mice. In comparison with a DNA fragment from the 5' region of the cloned AKR genome or one representing the entire genome, this selected probe hybridized to only a few MuLV proviruses. By comparing transformed and nontransformed tissue DNAs, it appeared that no amplification of proviral sequences related to the AKR ecotropic MuLV had occurred in thymomas of AKR mice during the development of leukemia in these animals. Analysis of the AKR ecotropic MuLV proviruses revealed a significant degree of polymorphism for these sequences among individuals in the AKR/J strain of mouse.

MeSH Terms
AKR murine leukemia virus/genetics Animals DNA, Viral/analysis Gene Amplification Genes, Viral Leukemia Virus, Murine/genetics Leukemia, Experimental/analysis Mice Mice, Inbred AKR Recombination, Genetic Thymoma/analysis Thymus Neoplasms/analysis
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yoshimura F K
Breda M
References (24)
24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.39.3.808-815.1981
Published
1981-09-00
Pages
808-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171313
Subset
IM
Grants
NCI NIH HHS · CA-25461 · United States
External Links
PubMed source DOI: 10.1128/JVI.39.3.808-815.1981 PMC Full Text View journal details
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