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PMID: 6270386 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Aberrant glycoprotein mRNA synthesized by the internal deletion mutant of vesicular stomatitis virus.

Journal of virology ·Vol. 40 ·No. 1 ·1981-10-00 ·Pages 78-86

Herman RC, Lazzarini RA

Abstract

The internal deletion mutant (DI-LT) derived from the heat-resistant strain of vesicular stomatitis virus synthesized an aberrant polyadenylated mRNA in vivo and in vitro. No normal glycoprotein message could be detected among the in vivo transcription products. The abnormal RNA contained a transcript of the partially deleted polymerase gene covalently linked to the 3' end of the glycoprotein message. The polyadenylate is located at the 3' end of the molecule and is most probably encoded by the remnant polymerase gene polyadenylation signal. This aberrant RNA may be synthesized because of either a failure to terminate transcription at the end of the glycoprotein gene or an inability to process an abnormal polycistronic precursor.

MeSH Terms
Animals Cell Line Chromosome Deletion Cricetinae Gene Expression Regulation Genes, Viral Glycoproteins/genetics Mutation Poly A/metabolism RNA, Messenger/genetics RNA-Dependent RNA Polymerase/genetics Transcription, Genetic Vesicular stomatitis Indiana virus/genetics Viral Proteins/genetics
Chemicals
Glycoproteins RNA, Messenger Viral Proteins Poly A RNA-Dependent RNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herman R C
Lazzarini R A
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45 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.1.78-86.1981
Published
1981-10-00
Pages
78-86
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256597
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.40.1.78-86.1981 PMC Full Text View journal details
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