Home LiteratureArticle Details
PMID: 6272321 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pleiotropic expression of Epstein--Barr virus DNA in human epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5852-5

Stoerker J, Parris D, Yajima Y, Glaser R

Abstract

We have attempted to establish a system that can be used to study the association of Epstein--Barr virus (EBV) with epithelial cells. Attempts were made to transfect human carcinoma cells with EBV DNA. Successful transfection was confirmed by the expression of EBV-specific early antigen (EA), virus capsid antigen, and the presence of virus DNA. The transfecting preparation contained a mixture of EBV and cellular DNA extracted from two producer cell lines, P3HR-1 and AG-876. Our data suggest that virus DNA obtained from the P3HR-1 nontransforming, EA-inducing strain of EBV was lytically expressed in the epithelial tumor cells. The DNA derived from AG-876 cells, which produce a transforming, non-EA-inducing strain of EBV, also produced a lytic infection.

MeSH Terms
Carcinoma/microbiology Cell Transformation, Viral Cells, Cultured DNA, Viral/genetics Epithelium/microbiology Gene Expression Regulation Genes, Viral Herpesvirus 4, Human/genetics Humans Nasopharyngeal Neoplasms/microbiology Transfection Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stoerker J
Parris D
Yajima Y
Glaser R
References (20)
20 references, click to expand
  1. Synthesis of Epstein-Barr virus antigens and DNA in activated Burkitt somatic cell hybrids.
    Virology. 1973 Sep;55(1):62-9 PMID: 4353957
  2. Immunofluorescence in cells derived from Burkitt's lymphoma.
    J Bacteriol. 1966 Mar;91(3):1248-56 PMID: 4160230
  3. Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells.
    J Virol. 1972 Aug;10(2):288-96 PMID: 4342246
  4. Transfection of human lymphoblastoid cells with herpes simplex viral DNA.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):949-53 PMID: 218224
  5. Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus.
    J Exp Med. 1973 Dec 1;138(6):1398-412 PMID: 4357683
  6. Precipitating antibody in human serum to an antigen present in cultured burkitt's lymphoma cells.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1699-704 PMID: 16591407
  7. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
    Int J Cancer. 1973 May;11(3):499-520 PMID: 4133943
  8. Cell surface markers on epithelial-Burkitt hybrid cells superinfected with Epstein-Barr virus.
    Cancer Res. 1977 Jul;37(7 Pt 1):2291-6 PMID: 193641
  9. Antibodies to Epstein-Barr virus in nasopharyngeal carcinoma, other head and neck neoplasms, and control groups.
    J Natl Cancer Inst. 1970 Jan;44(1):225-31 PMID: 11515035
  10. Effect of the host cell on the maintenance and replication of Epstein-Barr virus.
    Virology. 1976 Jan;69(1):132-42 PMID: 174288
  11. A new tumour-derived transforming strain of Epstein-Barr virus.
    Nature. 1978 Apr 13;272(5654):629-31 PMID: 205793
  12. Superinfection epithelial nasopharyngeal carcinoma cells with Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):960-3 PMID: 176664
  13. Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):433-6 PMID: 6244558
  14. Host cell regulation of induction of Epstein-Barr virus.
    J Virol. 1974 Jul;14(1):174-6 PMID: 4365330
  15. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA.
    Lancet. 1964 Mar 28;1(7335):702-3 PMID: 14107961
  16. Epstein-Barr viral DNA: infectivity for human placental cells.
    Science. 1981 Apr 24;212(4493):452-5 PMID: 6259735
  17. DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3265-8 PMID: 4361687
  18. Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection, and interference.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4006-10 PMID: 4372601
  19. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  20. Attempt to infect nonmalignant nasopharyngeal epithelial cells from humans and squirrel monkeys with Epstein-Barr virus.
    J Natl Cancer Inst. 1980 May;64(5):1085-90 PMID: 6245300
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5852
Published
1981-09-00
Pages
5852-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348885
Subset
IM
Grants
NCI NIH HHS · CA-16058 · United States
NCI NIH HHS · N01-CP-81021 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.9.5852 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2