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PMID: 6273852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 6139-43

Frink RJ, Draper KG, Wagner EK

Abstract

The sequences of the DNAs encoding the 5' ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5' ends of these mRNA species were precisely located. Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5' ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus.

MeSH Terms
Base Sequence Cell Transformation, Viral DNA Restriction Enzymes DNA, Viral/genetics DNA-Directed RNA Polymerases/metabolism Genes, Viral HeLa Cells/metabolism Humans Kinetics RNA, Messenger/genetics Simplexvirus/genetics Transcription, Genetic
Chemicals
DNA, Viral RNA, Messenger DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frink R J
Draper K G
Wagner E K
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.10.6139
Published
1981-10-00
Pages
6139-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348993
Subset
IM
Grants
NCI NIH HHS · CA11861 · United States
Databases
GENBANK
J02215, J02221
External Links
PubMed source DOI: 10.1073/pnas.78.10.6139 PMC Full Text View journal details
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