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PMID: 6275113 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains.

Journal of virology ·Vol. 40 ·No. 3 ·1981-12-00 ·Pages 720-8

Dyall-Smith ML, Holmes IH

Abstract

The polypeptides of SA11 rotavirus produced in virus-infected cells were analyzed by limited proteolysis, using Staphylococcus aureus V8 protease. This clearly distinguished between all the known primary gene products of the virus and allowed relationships between other infected-cell proteins, and between infected-cell and virus structural proteins, to be ascertained. A comparison of the proteolysis cleavage patterns between SA11 rotavirus and the human rotavirus Wa was also performed which demonstrated a marked conservation in the digestion patterns among nonstructural and inner-shell structural proteins, but a marked variation in the digestion patterns among outer-shell structural proteins.

MeSH Terms
Animals Cell Line Electrophoresis, Polyacrylamide Gel Glycoproteins/analysis Peptide Hydrolases Peptides/analysis Proteins/analysis Reoviridae/analysis Rotavirus/analysis Viral Proteins/analysis
Chemicals
Glycoproteins Peptides Proteins Viral Proteins Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dyall-Smith M L
Holmes I H
References (33)
33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.3.720-728.1981
Published
1981-12-00
Pages
720-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256683
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.40.3.720-728.1981 PMC Full Text View journal details
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