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PMID: 6276360 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 149 ·No. 2 ·1982-02-00 ·Pages 542-7

Kiss GB, Pearlman RE, Cornish KV, Friesen JD, Chan VL

Abstract

The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kinase coding sequence was detected.

MeSH Terms
Cloning, Molecular DNA Replication DNA, Recombinant Genes, Viral Plasmids Saccharomyces cerevisiae/genetics Simplexvirus/genetics Thymidine Kinase/genetics Transcription, Genetic Transformation, Genetic
Chemicals
DNA, Recombinant Thymidine Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kiss G B
Pearlman R E
Cornish K V
Friesen J D
Chan V L
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.149.2.542-547.1982
Published
1982-02-00
Pages
542-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216540
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.149.2.542-547.1982 PMC Full Text View journal details
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