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PMID: 6276549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone.

The Journal of membrane biology ·Vol. 64 ·No. 1-2 ·1982-00-00 ·Pages 77-89

Li JH, Palmer LG, Edelman IS, Lindemann B

Abstract

Urinary bladders of Bufo marinus were depolarized, by raising the serosal K concentration, to facilitate voltage-clamping of the apical membrane. Passive Na transport across the apical membrane was then studied with near-instantaneous current-voltage curves obtained before and after eliciting a natriferic response with oxytocin. Fitting with the constant-field equation showed that the natriferic effect is accounted for by an increase in the apical Na permeability. It is accompanied by a small increase in cellular Na activity. Furthermore, fluctuation analysis of the amiloride-induced shot-noise component of the short-circuit current indicated that the permeability increase is not due to increased Na translocation through those Na channels which were already conducting prior to hormonal stimulation. Rather, the natriferic effects is found to be based on an increase in the population of transporting channels. It appears that, in response to the hormone, Na channels are rapidly "recruited" from a pool of electrically silent channels.

MeSH Terms
Animals Biological Transport, Active/drug effects Bufo marinus Electric Conductivity Ion Channels/drug effects,physiology Kinetics Oxytocin/pharmacology Sodium/metabolism Urinary Bladder/drug effects,physiology
Chemicals
Ion Channels Oxytocin Sodium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li J H
Palmer L G
Edelman I S
Lindemann B
References (32)
32 references, click to expand
  1. Differential covalent labeling of apical and basal-lateral membranes of the epithelium of the toad bladder.
    J Membr Biol. 1976 Mar 18;26(2-3):301-17 PMID: 817032
  2. Direct visualization of epithelial morphology in the living amphibian urinary bladder.
    J Membr Biol. 1978;40 Spec No:45-70 PMID: 104042
  3. Quantitative analysis of exocytosis and endocytosis in the hydroosmotic response of toad bladder.
    J Membr Biol. 1980;52(3):221-35 PMID: 6770096
  4. Low-noise amplification of voltage and current fluctuations arising in epithelia.
    Rev Sci Instrum. 1978 Jan;49(1):52 PMID: 18698937
  5. The effect of Ca and antidiuretic hormone on Na transport across frog skin. II. Sites and mechanisms of action.
    J Gen Physiol. 1963 May;46:1011-27 PMID: 14024308
  6. Sodium transport across toad urinary bladder: a model "tight" epithelium.
    Physiol Rev. 1980 Jul;60(3):615-715 PMID: 6248906
  7. Solvent drag on non-electrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone.
    Acta Physiol Scand. 1957 Jun 8;39(2-3):228-39 PMID: 13444038
  8. Mass transport across cell membranes: the effects of antidiuretic hormone on water and solute flows in epithelia.
    Annu Rev Physiol. 1976;38:451-500 PMID: 176921
  9. Amiloride and the sodium channel.
    Naunyn Schmiedebergs Arch Pharmacol. 1974;281(3):261-9 PMID: 4275046
  10. Sodium-specific membrane channels of frog skin are pores: current fluctuations reveal high turnover.
    Science. 1977 Jan 21;195(4275):292-4 PMID: 299785
  11. Concentration dependence of currents through single sodium-selective pores in frog skin.
    Nature. 1979 Nov 29;282(5738):519-20 PMID: 315521
  12. [Action characteristics of a new acylguanidine--amiloride-HCl (MK 870)--on the isolated skin of amphibia].
    Klin Wochenschr. 1967 Jul 15;45(14):737-8 PMID: 5592444
  13. Effects of pH, Ca, ADH, and theophylline on kinetics of Na entry in frog skin.
    Am J Physiol. 1978 Jul;235(1):C35-48 PMID: 27988
  14. A characteristic response of the isolated frog skin potential to neurohypophysial principles and its relation to the transport of sodium and water.
    J Cell Comp Physiol. 1951 Aug;38(1):109-30 PMID: 14873760
  15. Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.
    J Clin Invest. 1977 Mar;59(3):576-81 PMID: 402387
  16. Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.
    J Physiol. 1977 May;267(1):137-66 PMID: 301566
  17. Current-voltage analysis of apical sodium transport in toad urinary bladder: effects of inhibitors of transport and metabolism.
    J Membr Biol. 1980 Nov 15;57(1):59-71 PMID: 6256553
  18. Control of apical sodium permeability in the toad urinary bladder by aldosterone.
    Ann N Y Acad Sci. 1981;372:1-14 PMID: 6176162
  19. On the use of general network functions in the evaluation of noise spectra obtained from epithelia.
    Soc Gen Physiol Ser. 1981;36:1-13 PMID: 6269225
  20. Capacitance changes in frog skin caused by theophylline and antidiuretic hormone.
    Br J Pharmacol. 1969 Sep;37(1):314-24 PMID: 5343355
  21. Estimation of the density of sodium entry sites in frog skin epithelium from the uptake of [3H]benzamil.
    J Physiol. 1979 Oct;295:477-90 PMID: 316450
  22. THE INFLUENCE OF NA CONCENTRATION ON NA TRANSPORT ACROSS FROG SKIN.
    J Gen Physiol. 1964 May;47:879-93 PMID: 14155434
  23. Amiloride as a probe for sodium entry sites in frog skin epithelium [proceedings].
    J Physiol. 1977 Mar;266(1):28P-29P PMID: 300802
  24. Aldosterone control of the density of sodium channels in the toad urinary bladder.
    J Membr Biol. 1982;64(1-2):91-102 PMID: 6276550
  25. The role of adenosine 3',5'-phosphate in the action of antidiuretic hormone.
    Am J Med. 1967 May;42(5):757-68 PMID: 5337377
  26. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.
    J Gen Physiol. 1962 Jan;45:529-43 PMID: 13894805
  27. Evidence that ADH-stimulated intramembrane particle aggregates are transferred from cytoplasmic to luminal membranes in toad bladder epithelial cells.
    J Cell Biol. 1980 Apr;85(1):83-95 PMID: 6767731
  28. Membrane structural specialization of the toad urinary bladder revealed by the freeze-fracture technique. III. Location, structure and vasopressin dependence of intramembrane particle arrays.
    J Membr Biol. 1978;40 Spec No:281-96 PMID: 104039
  29. Effect of antidiuretic hormone on sodium uptake across outer surface of frog skin.
    Am J Physiol. 1973 Oct;225(4):912-7 PMID: 4542709
  30. Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells.
    Proc R Soc Lond B Biol Sci. 1975 Jun 17;189(1097):543-75 PMID: 237282
  31. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder.
    J Clin Invest. 1962 Apr;41:702-9 PMID: 14482265
  32. How many conductance states do potassium channels have?
    Biophys J. 1975 Aug;15(8):843-6 PMID: 1148365
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870770
Published
1982-00-00
Pages
77-89
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIADDK NIH HHS · AM-07219 · United States
NIADDK NIH HHS · AM-13659 · United States
NHLBI NIH HHS · HL-S3115 · United States
External Links
PubMed source DOI: 10.1007/BF01870770 View journal details
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