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PMID: 6770096 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Quantitative analysis of exocytosis and endocytosis in the hydroosmotic response of toad bladder.

The Journal of membrane biology ·Vol. 52 ·No. 3 ·1980-00-00 ·Pages 221-35

Gronowicz G, Masur SK, Holtzman E

Abstract

This study concerns the timing and magnitude of exocytosis and endocytosis in the granular cells of toad bladder during the hydroosmotic response to antidiuretic hormone. Granule exocytosis at the luminal cell surface is extensive within 5 min of the administration of a physiological dose of hormone. Hydroosmosis becomes detectable during this time period. The amount of membrane added to the luminal surface by exocytosis during 60 min of exposure to hormone can be of the same order of magnitude as the extent of the luminal plasma membrane. Endocytosis, demonstrated by peroxidase uptake from the luminal surface, becomes extensive during the period 15-45 min after hormone administration. Thus, maximal endocytic activity occurs later than the period of most extensive exocytosis and seems to correlate with the onset of the decline in water movement. The amount of membrane retrieved from the luminal surface by endocytosis during 60 min of stimulation is at least three quarters of that added by exocytosis. The bulk membrane movement in ADH stimulated preparations does not require the presence of an osmotic gradient. Colchicine inhibits the hydroosmotic response, the exocytosis of granules, and endocytosis at the luminal surface. These results strengthen our view that the bulk circulation of membrane at the cell surface, via exocytosis and endocytosis, is closely related to the permeability changes occurring at the surface.

MeSH Terms
Animals Bufo marinus Cell Membrane Permeability/drug effects Colchicine/pharmacology Cytoplasmic Granules/physiology Endocytosis/drug effects Exocytosis/drug effects Female Microscopy, Electron Osmosis/drug effects Potassium/pharmacology Time Factors Urinary Bladder/drug effects,physiology,ultrastructure Vasopressins/pharmacology
Chemicals
Vasopressins Potassium Colchicine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gronowicz G
Masur S K
Holtzman E
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64 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869191
Published
1980-00-00
Pages
221-35
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869191 View journal details
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