Home LiteratureArticle Details

PMID: 6278241 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.

Molecular & general genetics : MGG ·Vol. 183 ·No. 3 ·1981-00-00 ·Pages 497-504

Willis DK, Uhlin BE, Amini KS, Clark AJ

Abstract

A restriction endonuclease map for the enzymes EcoRI, BamHI, SalI, and PstI covering 23.5 kilobase pairs (kb) of the srl recA region of Escherichia coli was constructed. An insertion of the transposon Tn10 in the negative regulatory gene srlR was shown to be located 5.8 kb away from the promoter proximal end of the recA gene. The extent of several Tn10 generated deletions, originating from the srlR301::Tn10 insertion, were analyzed by physical mapping. Three mutations that had removed the Tn10 encoded tetracycline resistance gene, del(srl-recA)302, del(srl-recA)304, and del(srl-recA)303, were found to be deleted for 40%, 45%, and 50% of the recA structural gene, respectively. A deletion, del(srl-recA)306, that had not affected the structure of the Tn10 in srlR301 was shown to have removed the entire recA structural gene.

MeSH Terms

Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes/metabolism DNA Transposable Elements DNA, Bacterial Escherichia coli/genetics Genes, Bacterial Mutation Recombination, Genetic

Chemicals

DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Willis D K

Uhlin B E

Amini K S

Clark A J

References (27)

27 references, click to expand

Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.
J Bacteriol. 1980 Jul;143(1):529-30 PMID: 6249792
Physical characterisation of the "Rac prophage" in E. coli K12.
Mol Gen Genet. 1979 Sep;175(2):159-74 PMID: 390313
Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.
J Bacteriol. 1975 Oct;124(1):26-38 PMID: 1100602
Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
Organization of the recA gene of Escherichia coli.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):313-7 PMID: 6244554
Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance.
J Bacteriol. 1979 Jan;137(1):681-5 PMID: 762025
Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
J Bacteriol. 1981 Jan;145(1):521-32 PMID: 6257642
[Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation].
Mol Gen Genet. 1972;119(2):153-74 PMID: 4577433
A new method for the purification and identification of covalently closed circular DNA molcules.
Nucleic Acids Res. 1978 Apr;5(4):1139-52 PMID: 26042
Characteristics of purified recA protein and the regulation of its synthesis in vivo.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:909-15 PMID: 158476
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
Genetics. 1978 Nov;90(3):427-61 PMID: 365678
Genetic analysis of the Escherichia coli K-12 srl region.
J Bacteriol. 1977 Dec;132(3):904-11 PMID: 336611
A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
Cell. 1976 Sep;9(1):91-9 PMID: 788919
Physical map of the recA gene.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3144-8 PMID: 386332
Recombination deficient mutants of E. coli and other bacteria.
Annu Rev Genet. 1973;7:67-86 PMID: 4205905
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Genetic organization of transposon Tn10.
Cell. 1981 Jan;23(1):201-13 PMID: 6260375
Plasmids with temperature-dependent copy number for amplification of cloned genes and their products.
Gene. 1979 Jun;6(2):91-106 PMID: 383579
The genetic characterization of lexB32, lexB33 and lexB35 mutations of Escherichia coli: location and complementation pattern for UV resistance.
Mol Gen Genet. 1977 Nov 29;157(1):83-9 PMID: 340915
Identification of the recA (tif) gene product of Escherichia coli.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5280-4 PMID: 341152
Translocation and other recombination events involving the tetracycline-resistance element Tn10.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1233-46 PMID: 158473
Isolation and characterization of specialized transducing bacteriophages for the recA gene of Escherichia coli.
Virology. 1977 Mar;77(1):306-18 PMID: 320757
Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.
J Bacteriol. 1979 Jun;138(3):705-14 PMID: 378932
Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.
Genetics. 1979 Oct;93(2):321-43 PMID: 395024
Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetitions.
Cell. 1979 Apr;16(4):721-31 PMID: 455446
Cloning, partial sequencing, and in vitro transcription of the gene for alanine tRNA synthetase.
J Biol Chem. 1981 Jan 10;256(1):205-11 PMID: 6256345
Sequences of the recA gene and protein.
Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5 PMID: 6930655

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268771

Published

1981-00-00

Pages

497-504

Language

English

Region

Germany

NLM ID

0125036

Subset

Grants

NIAID NIH HHS · AI05371 · United States

NIGMS NIH HHS · GM07232 · United States

External Links

PubMed source DOI: 10.1007/BF00268771

Analysis Services

Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.

Contact

WeChat Official Account