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PMID: 386332 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical map of the recA gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3144-8

Sancar A, Rupp WD

Abstract

We have cloned the recA gene of Echerichia coli K12 and some of its restriction fragments on the plasmid cloning vehicle pBR322. The recA gene was mapped with regard to the restriction sites of EcoRI, BamHI, Pst I, Hha I, Hae III, HinfI, and Taq I restriction endonucleases. The recA promoter was localized by the binding of RNA polymerase to restriction fragments. The initiation point of transcription of recA mRNA and the direction of transcription were determined from in vitro transcription of recA gene fragments and from analysis of the polypeptides made in maxicells that contain plasmids carrying only part of the recA gene.

MeSH Terms

Bacterial Proteins/genetics Chromosome Mapping DNA Restriction Enzymes DNA, Bacterial/genetics DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Genes Plasmids Transcription, Genetic

Chemicals

Bacterial Proteins DNA, Bacterial DNA-Directed RNA Polymerases DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sancar A

Rupp W D

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.7.3144

Published

1979-07-00

Pages

3144-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383780

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.7.3144 PMC Full Text View journal details

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