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PMID: 6278416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The 5S RNA genes of Schizosaccharomyces pombe.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 487-500

Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D

Abstract

The genomic arrangement and sequences of S. pombe 5S RNA genes are reported here. The 5S gene sequences appear to be dispersed within the genome, and are found independently of other rRNA genes. The sequences of two 5S genes examined show identical coding regions of 119 base pairs but have widely varying flanking sequences. A tRNAAsp gene is found in the 3' flanking region of one of the 5S genes. The tRNAAsp gene is faithfully transcribed in an X. laevis in vitro system, while the 5S genes are not transcribed in this system. The phylogenetic position of S. pombe is examined through comparison of 5S RNA sequences.

MeSH Terms
Animals Ascomycota/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/genetics Genes Nucleic Acid Conformation Plasmids RNA, Ribosomal/genetics Schizosaccharomyces/genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mao J
Appel B
Schaack J
Sharp S
Yamada H
Söll D
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.487
Published
1982-01-22
Pages
487-500
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326152
Subset
IM
Databases
GENBANK
K00569, K00570
External Links
PubMed source DOI: 10.1093/nar/10.2.487 PMC Full Text View journal details
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