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PMID: 6278494 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 3 ·1982-02-00 ·Pages 790-4

Wettstein FO, Stevens JG

Abstract

The state of rabbit (Shope) papilloma virus DNA in virus-induced nonproducing tumors on domestic rabbits was investigated. Virus-specific sequences were resolved into many distinct bands by one-dimensional agarose gel electrophoresis. Two-dimensional gel electrophoresis, CsCl/propidium iodide density equilibrium centrifugation, partial digestion with a restriction endonuclease, and S1 nuclease digestion permitted us to identify the bands as free viral episomes representing circular molecules of increasing size. In some tumors (both papillomas and carcinomas), up to 25% of the virus-specific DNA was linear and comigrated with cellular DNA. Integration of at least some of these sequences was suggested by the detection of viral-cellular junction bands in one tumor after digestion of DNA with EcoRI and Sal I, enzymes that cut Shope DNA once. Finally, the physical states of viral DNA in papillomas and carcinomas were found to be similar, although free episomes were generally larger in carcinomas.

MeSH Terms
Animals Cell Transformation, Viral Chromosomes/ultrastructure Cottontail rabbit papillomavirus/genetics DNA, Superhelical/analysis DNA, Viral/metabolism Molecular Weight Papillomaviridae/genetics Plasmids Rabbits Recombination, Genetic Tumor Virus Infections/genetics Virus Replication
Chemicals
DNA, Superhelical DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wettstein F O
Stevens J G
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.3.790
Published
1982-02-00
Pages
790-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345838
Subset
IM
Grants
NCI NIH HHS · CA-18151 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.3.790 PMC Full Text View journal details
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